This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Abcam
other brands :
Epitomics, Ascent Scientific, MitoSciences
product type :
antibody
product name :
Anti-CENPA antibody [3-19] - ChIP Grade
catalog :
ab13939
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3-19
reactivity :
human, mouse
application :
western blot, immunocytochemistry, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 74
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:300; loading ...; fig 3e
Soto M, García Santisteban I, Krenning L, Medema R, Raaijmakers J. Chromosomes trapped in micronuclei are liable to segregation errors. J Cell Sci. 2018;131: pubmed publisher
  • immunocytochemistry; human; 1:500; loading ...; fig 6e
Despras E, Sittewelle M, Pouvelle C, Delrieu N, Cordonnier A, Kannouche P. Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA. Nat Commun. 2016;7:13326 pubmed publisher
  • immunocytochemistry; human; 1:500
Nixon F, Gutiérrez Caballero C, Hood F, Booth D, Prior I, Royle S. The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. elife. 2015;4: pubmed publisher
  • immunocytochemistry; human; 1:400; fig 5
Prosser S, Sahota N, Pelletier L, Morrison C, Fry A. Nek5 promotes centrosome integrity in interphase and loss of centrosome cohesion in mitosis. J Cell Biol. 2015;209:339-48 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 13
Zahn J, Kaplan M, Fischer S, Dai M, Meng F, Saha A, et al. Expansion of a novel endogenous retrovirus throughout the pericentromeres of modern humans. Genome Biol. 2015;16:74 pubmed publisher
  • chromatin immunoprecipitation; human; fig 1
  • immunocytochemistry; human; fig 8
  • western blot; human; fig 1
Athwal R, Walkiewicz M, Baek S, Fu S, Bui M, Camps J, et al. CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells. Epigenetics Chromatin. 2015;8:2 pubmed publisher
  • chromatin immunoprecipitation; human
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:200
González Barrios R, Soto Reyes E, Quiroz Baez R, Fabián Morales E, Díaz Chávez J, Del Castillo V, et al. Differential distribution of HP1 proteins after trichostatin a treatment influences chromosomal stability in HCT116 and WI-38 cells. Cell Div. 2014;9:6 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3c
Espert A, Uluocak P, Bastos R, Mangat D, Graab P, Gruneberg U. PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing. J Cell Biol. 2014;206:833-42 pubmed publisher
  • immunocytochemistry; human; fig 2c
Shandilya J, Toska E, Richard D, Medler K, Roberts S. WT1 interacts with MAD2 and regulates mitotic checkpoint function. Nat Commun. 2014;5:4903 pubmed publisher
  • immunocytochemistry; human; fig s10
Smith C, Matheson T, Trombly D, Sun X, Campeau E, Han X, et al. A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli. Mol Biol Cell. 2014;25:2866-81 pubmed publisher
  • immunocytochemistry; human
Mäki Jouppila J, Laine L, Rehnberg J, Narvi E, Tiikkainen P, Hukasova E, et al. Centmitor-1, a novel acridinyl-acetohydrazide, possesses similar molecular interaction field and antimitotic cellular phenotype as rigosertib, on 01910.Na. Mol Cancer Ther. 2014;13:1054-66 pubmed publisher
  • immunocytochemistry; human; 1:500
Stimpson K, Sullivan L, Kuo M, Sullivan B. Nucleolar organization, ribosomal DNA array stability, and acrocentric chromosome integrity are linked to telomere function. PLoS ONE. 2014;9:e92432 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100
  • western blot; human; 1:500
Gu X, Fu J, Feng X, Huang X, Wang S, Chen X, et al. Expression and prognostic relevance of centromere protein A in primary osteosarcoma. Pathol Res Pract. 2014;210:228-33 pubmed publisher
  • western blot; human
Hofmann J, Tegha Dunghu J, Dräger S, Will C, Luhrmann R, Gruss O. The Prp19 complex directly functions in mitotic spindle assembly. PLoS ONE. 2013;8:e74851 pubmed publisher
  • immunohistochemistry - paraffin section; human
Orecchioni S, Gregato G, Martin Padura I, Reggiani F, Braidotti P, Mancuso P, et al. Complementary populations of human adipose CD34+ progenitor cells promote growth, angiogenesis, and metastasis of breast cancer. Cancer Res. 2013;73:5880-91 pubmed publisher
Murillo Pineda M, Valente L, Dumont M, Mata J, Fachinetti D, Jansen L. Induction of spontaneous human neocentromere formation and long-term maturation. J Cell Biol. 2021;220: pubmed publisher
Dopie J, Sweredoski M, Moradian A, Belmont A. Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins. J Cell Biol. 2020;219: pubmed publisher
Swartz S, McKay L, Su K, Bury L, Padeganeh A, Maddox P, et al. Quiescent Cells Actively Replenish CENP-A Nucleosomes to Maintain Centromere Identity and Proliferative Potential. Dev Cell. 2019;51:35-48.e7 pubmed publisher
Logsdon G, Gambogi C, Liskovykh M, Barrey E, Larionov V, Miga K, et al. Human Artificial Chromosomes that Bypass Centromeric DNA. Cell. 2019;178:624-639.e19 pubmed publisher
Leimbacher P, Jones S, Shorrocks A, De Marco Zompit M, Day M, Blaauwendraad J, et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Mol Cell. 2019;74:571-583.e8 pubmed publisher
Ly P, Brunner S, Shoshani O, Kim D, Lan W, Pyntikova T, et al. Chromosome segregation errors generate a diverse spectrum of simple and complex genomic rearrangements. Nat Genet. 2019;51:705-715 pubmed publisher
Kyriacou E, Heun P. High-resolution mapping of centromeric protein association using APEX-chromatin fibers. Epigenetics Chromatin. 2018;11:68 pubmed publisher
Lee H, Lin Z, Chae S, Yoo Y, Kim B, Lee Y, et al. The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation. Nat Commun. 2018;9:3848 pubmed publisher
Pesenti M, Prumbaum D, Auckland P, Smith C, Faesen A, Petrovic A, et al. Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80. Mol Cell. 2018;71:923-939.e10 pubmed publisher
Fielding A, Concannon M, Darling S, Rusilowicz Jones E, Sacco J, Prior I, et al. The deubiquitylase USP15 regulates topoisomerase II alpha to maintain genome integrity. Oncogene. 2018;37:2326-2342 pubmed publisher
Zhao Y, Ye S, Liang D, Wang P, Fu J, Ma Q, et al. In Vitro Modeling of Human Germ Cell Development Using Pluripotent Stem Cells. Stem Cell Reports. 2018;10:509-523 pubmed publisher
Thakur J, Henikoff S. Unexpected conformational variations of the human centromeric chromatin complex. Genes Dev. 2018;32:20-25 pubmed publisher
Chen J, Teo B, Cai Y, Wee S, Lu J. The linker histone H1.2 is a novel component of the nucleolar organizer regions. J Biol Chem. 2018;293:2358-2369 pubmed publisher
Gangula N, Maddika S. Interplay between the phosphatase PHLPP1 and E3 ligase RNF41 stimulates proper kinetochore assembly via the outer-kinetochore protein SGT1. J Biol Chem. 2017;292:13947-13958 pubmed publisher
Auckland P, Clarke N, Royle S, McAinsh A. Congressing kinetochores progressively load Ska complexes to prevent force-dependent detachment. J Cell Biol. 2017;216:1623-1639 pubmed publisher
Barnhart Dailey M, Trivedi P, Stukenberg P, Foltz D. HJURP interaction with the condensin II complex during G1 promotes CENP-A deposition. Mol Biol Cell. 2017;28:54-64 pubmed publisher
Myrianthopoulos V, Gaboriaud Kolar N, Tallant C, Hall M, Grigoriou S, Brownlee P, et al. Discovery and Optimization of a Selective Ligand for the Switch/Sucrose Nonfermenting-Related Bromodomains of Polybromo Protein-1 by the Use of Virtual Screening and Hydration Analysis. J Med Chem. 2016;59:8787-8803 pubmed
Ross J, Woodlief K, Sullivan B. Inheritance of the CENP-A chromatin domain is spatially and temporally constrained at human centromeres. Epigenetics Chromatin. 2016;9:20 pubmed publisher
Minocherhomji S, Ying S, Bjerregaard V, Bursomanno S, Aleliunaite A, Wu W, et al. Replication stress activates DNA repair synthesis in mitosis. Nature. 2015;528:286-90 pubmed publisher
Xiao T, Wongtrakoongate P, TRAINOR C, Felsenfeld G. CTCF Recruits Centromeric Protein CENP-E to the Pericentromeric/Centromeric Regions of Chromosomes through Unusual CTCF-Binding Sites. Cell Rep. 2015;12:1704-14 pubmed publisher
Holmberg Olausson K, Nistér M, Lindström M. Loss of nucleolar histone chaperone NPM1 triggers rearrangement of heterochromatin and synergizes with a deficiency in DNA methyltransferase DNMT3A to drive ribosomal DNA transcription. J Biol Chem. 2014;289:34601-19 pubmed publisher
Walkiewicz M, Bui M, Quenet D, Dalal Y. Tracking histone variant nucleosomes across the human cell cycle using biophysical, biochemical, and cytological analyses. Methods Mol Biol. 2014;1170:589-615 pubmed publisher
Wilson J, Tejera A, Castor D, Toth R, Blasco M, Rouse J. Localization-dependent and -independent roles of SLX4 in regulating telomeres. Cell Rep. 2013;4:853-60 pubmed publisher
Hasson D, Panchenko T, Salimian K, Salman M, Sekulic N, Alonso A, et al. The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013;20:687-95 pubmed publisher
Maloney K, Sullivan L, Matheny J, Strome E, Merrett S, Ferris A, et al. Functional epialleles at an endogenous human centromere. Proc Natl Acad Sci U S A. 2012;109:13704-9 pubmed publisher
Rouzeau S, Cordelières F, Buhagiar Labarchède G, Hurbain I, Onclercq Delic R, Gemble S, et al. Bloom's syndrome and PICH helicases cooperate with topoisomerase II? in centromere disjunction before anaphase. PLoS ONE. 2012;7:e33905 pubmed publisher
Wu Q, Qian Y, Zhao X, Wang S, Feng X, Chen X, et al. Expression and prognostic significance of centromere protein A in human lung adenocarcinoma. Lung Cancer. 2012;77:407-14 pubmed publisher
Slatter T, Tan X, Yuen Y, Gunningham S, Ma S, Daly E, et al. The alternative lengthening of telomeres pathway may operate in non-neoplastic human cells. J Pathol. 2012;226:509-18 pubmed publisher
Slee R, Steiner C, Herbert B, Vance G, Hickey R, Schwarz T, et al. Cancer-associated alteration of pericentromeric heterochromatin may contribute to chromosome instability. Oncogene. 2012;31:3244-53 pubmed publisher
Liu L, Li Y, Zhang S, Yu D, Zhu M. Hepatitis B virus X protein mutant upregulates CENP-A expression in hepatoma cells. Oncol Rep. 2012;27:168-73 pubmed publisher
Barnhart M, Kuich P, Stellfox M, Ward J, Bassett E, Black B, et al. HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore. J Cell Biol. 2011;194:229-43 pubmed publisher
Li Y, Zhu Z, Zhang S, Yu D, Yu H, Liu L, et al. ShRNA-targeted centromere protein A inhibits hepatocellular carcinoma growth. PLoS ONE. 2011;6:e17794 pubmed publisher
Barrett R, Colnaghi R, Wheatley S. Threonine 48 in the BIR domain of survivin is critical to its mitotic and anti-apoptotic activities and can be phosphorylated by CK2 in vitro. Cell Cycle. 2011;10:538-48 pubmed
Zeng K, Bastos R, Barr F, Gruneberg U. Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2. J Cell Biol. 2010;191:1315-32 pubmed publisher
Lee S, Itkin Ansari P, Levine F. CENP-A, a protein required for chromosome segregation in mitosis, declines with age in islet but not exocrine cells. Aging (Albany NY). 2010;2:785-90 pubmed
Stimpson K, Song I, Jauch A, Holtgreve Grez H, Hayden K, Bridger J, et al. Telomere disruption results in non-random formation of de novo dicentric chromosomes involving acrocentric human chromosomes. PLoS Genet. 2010;6: pubmed publisher
Amaro A, Samora C, Holtackers R, Wang E, Kingston I, Alonso M, et al. Molecular control of kinetochore-microtubule dynamics and chromosome oscillations. Nat Cell Biol. 2010;12:319-29 pubmed publisher
Macurek L, Lindqvist A, Voets O, Kool J, Vos H, Medema R. Wip1 phosphatase is associated with chromatin and dephosphorylates gammaH2AX to promote checkpoint inhibition. Oncogene. 2010;29:2281-91 pubmed publisher
Pegoraro G, Kubben N, Wickert U, Göhler H, Hoffmann K, Misteli T. Ageing-related chromatin defects through loss of the NURD complex. Nat Cell Biol. 2009;11:1261-7 pubmed publisher
Mravinac B, Sullivan L, Reeves J, Yan C, Kopf K, Farr C, et al. Histone modifications within the human X centromere region. PLoS ONE. 2009;4:e6602 pubmed publisher
Markaki Y, Christogianni A, Politou A, Georgatos S. Phosphorylation of histone H3 at Thr3 is part of a combinatorial pattern that marks and configures mitotic chromatin. J Cell Sci. 2009;122:2809-19 pubmed publisher
Bergman L, Birts C, Darley M, Gabrielli B, Blaydes J. CtBPs promote cell survival through the maintenance of mitotic fidelity. Mol Cell Biol. 2009;29:4539-51 pubmed publisher
Buch C, Lindberg R, Figueroa R, Gudise S, Onischenko E, Hallberg E. An integral protein of the inner nuclear membrane localizes to the mitotic spindle in mammalian cells. J Cell Sci. 2009;122:2100-7 pubmed publisher
Klebig C, Korinth D, Meraldi P. Bub1 regulates chromosome segregation in a kinetochore-independent manner. J Cell Biol. 2009;185:841-58 pubmed publisher
Naim V, Rosselli F. The FANC pathway and BLM collaborate during mitosis to prevent micro-nucleation and chromosome abnormalities. Nat Cell Biol. 2009;11:761-8 pubmed publisher
Hong K, Kim H, Kim H, Seong Y, Hong K, Bae C, et al. Cdk1-cyclin B1-mediated phosphorylation of tumor-associated microtubule-associated protein/cytoskeleton-associated protein 2 in mitosis. J Biol Chem. 2009;284:16501-12 pubmed publisher
Kishi K, van Vugt M, Okamoto K, Hayashi Y, Yaffe M. Functional dynamics of Polo-like kinase 1 at the centrosome. Mol Cell Biol. 2009;29:3134-50 pubmed publisher
Moralli D, Chan D, Jefferson A, Volpi E, Monaco Z. HAC stability in murine cells is influenced by nuclear localization and chromatin organization. BMC Cell Biol. 2009;10:18 pubmed publisher
Kong X, Ball A, Sonoda E, Feng J, Takeda S, Fukagawa T, et al. Cohesin associates with spindle poles in a mitosis-specific manner and functions in spindle assembly in vertebrate cells. Mol Biol Cell. 2009;20:1289-301 pubmed publisher
Metzler Guillemain C, Depetris D, Luciani J, Mignon Ravix C, Mitchell M, Mattei M. In human pachytene spermatocytes, SUMO protein is restricted to the constitutive heterochromatin. Chromosome Res. 2008;16:761-82 pubmed publisher
Kondo Y, Shen L, Ahmed S, Boumber Y, Sekido Y, Haddad B, et al. Downregulation of histone H3 lysine 9 methyltransferase G9a induces centrosome disruption and chromosome instability in cancer cells. PLoS ONE. 2008;3:e2037 pubmed publisher
Montembault E, Dutertre S, Prigent C, Giet R. PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores. J Cell Biol. 2007;179:601-9 pubmed
Wang L, Schwarzbraun T, Speicher M, Nigg E. Persistence of DNA threads in human anaphase cells suggests late completion of sister chromatid decatenation. Chromosoma. 2008;117:123-35 pubmed
Biermann K, Heukamp L, Steger K, Zhou H, Franke F, Guetgemann I, et al. Gene expression profiling identifies new biological markers of neoplastic germ cells. Anticancer Res. 2007;27:3091-100 pubmed
Spence J, Phua H, Mills W, Carpenter A, Porter A, Farr C. Depletion of topoisomerase IIalpha leads to shortening of the metaphase interkinetochore distance and abnormal persistence of PICH-coated anaphase threads. J Cell Sci. 2007;120:3952-64 pubmed
Griffis E, Stuurman N, Vale R. Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. J Cell Biol. 2007;177:1005-15 pubmed
Morency E, Sabra M, Catez F, Texier P, Lomonte P. A novel cell response triggered by interphase centromere structural instability. J Cell Biol. 2007;177:757-68 pubmed
Dai J, Sullivan B, Higgins J. Regulation of mitotic chromosome cohesion by Haspin and Aurora B. Dev Cell. 2006;11:741-50 pubmed
Lam A, Boivin C, Bonney C, Rudd M, Sullivan B. Human centromeric chromatin is a dynamic chromosomal domain that can spread over noncentromeric DNA. Proc Natl Acad Sci U S A. 2006;103:4186-91 pubmed
product information
CatalogNumber :
ab13939
ItemName :
Anti-CENPA antibody [3-19] - ChIP Grade
Product Type :
Antibody
Host Species :
Mouse
Clonality :
Monoclonal
Conjugate :
Unconjugated
Clone Name :
3-19
Size :
50 µg
SpeciesSummary :
Hu
Purity :
Protein A purified
Application Summary :
ChIP, Flow Cyt, ICC/IF, IHC-P, WB
company information
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB20AX
https://www.abcam.com
888-7722226
headquarters: UK