This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Abcam
other brands :
Epitomics, Ascent Scientific, MitoSciences
product type :
antibody
product name :
Anti-Nucleolin antibody [4E2] - ChIP Grade
catalog :
ab13541
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
4E2
reactivity :
African green monkey, human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 48
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:5000; loading ...; fig 4a
Zaja R, Aydin G, Lippok B, Feederle R, Luscher B, Feijs K. Comparative analysis of MACROD1, MACROD2 and TARG1 expression, localisation and interactome. Sci Rep. 2020;10:8286 pubmed publisher
  • immunohistochemistry; human; loading ...; fig 1f
Xing Y, Yao R, Zhang Y, Guo C, Jiang S, Xu G, et al. SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Cell. 2017;169:664-678.e16 pubmed publisher
  • western blot; human; fig 5
Mori F, Ferraiuolo M, Santoro R, Sacconi A, Goeman F, Pallocca M, et al. Multitargeting activity of miR-24 inhibits long-term melatonin anticancer effects. Oncotarget. 2016;7:20532-48 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 9
Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Llères D, et al. The cell proliferation antigen Ki-67 organises heterochromatin. elife. 2016;5:e13722 pubmed publisher
  • western blot; human
Li M, Pehar M, Liu Y, Bhattacharyya A, Zhang S, O Riordan K, et al. The amyloid precursor protein (APP) intracellular domain regulates translation of p44, a short isoform of p53, through an IRES-dependent mechanism. Neurobiol Aging. 2015;36:2725-36 pubmed publisher
  • immunocytochemistry; human
Hofmann B, Schlüter L, Lange P, Mercanoglu B, Ewald F, Fölster A, et al. COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer. Mol Cancer. 2015;14:109 pubmed publisher
  • immunocytochemistry; human
Sokka M, Rilla K, Miinalainen I, Pospiech H, Syväoja J. High levels of TopBP1 induce ATR-dependent shut-down of rRNA transcription and nucleolar segregation. Nucleic Acids Res. 2015;43:4975-89 pubmed publisher
  • immunocytochemistry; human; fig 1
Katagiri N, Kuroda T, Kishimoto H, Hayashi Y, Kumazawa T, Kimura K. The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci Rep. 2015;5:8903 pubmed publisher
  • immunocytochemistry; human; fig s9
Warren D, Tajsic T, Porter L, Minaisah R, Cobb A, Jacob A, et al. Nesprin-2-dependent ERK1/2 compartmentalisation regulates the DNA damage response in vascular smooth muscle cell ageing. Cell Death Differ. 2015;22:1540-50 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:2000
Kong L, Wei J, Haider A, Liebelt B, Ling X, Conrad C, et al. Therapeutic targets in subependymoma. J Neuroimmunol. 2014;277:168-75 pubmed publisher
  • immunocytochemistry; human; 1:500
  • immunocytochemistry; African green monkey; 1:500
Turpin J, Journo C, Ko N, Sinet F, Carpentier A, Galioot A, et al. Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses. J Virol. 2015;89:931-51 pubmed publisher
  • immunocytochemistry; human
  • western blot; human
Peltonen K, Colis L, Liu H, Jäämaa S, Zhang Z, Af Hällström T, et al. Small molecule BMH-compounds that inhibit RNA polymerase I and cause nucleolar stress. Mol Cancer Ther. 2014;13:2537-46 pubmed publisher
  • western blot; human; 1:1000
Su L, Zheng H, Li Z, Qiu J, Chen S, Liu J, et al. Mechanistic studies on the anticancer activity of 2,4-disubstituted quinazoline derivative. Biochim Biophys Acta. 2014;1840:3123-30 pubmed publisher
  • western blot; human
Wakai M, Abe S, Kazuki Y, Oshimura M, Ishikawa F. A human artificial chromosome recapitulates the metabolism of native telomeres in mammalian cells. PLoS ONE. 2014;9:e88530 pubmed publisher
Cudalbu C, Bady P, Lai M, Xin L, Gusyatiner O, Hamou M, et al. Metabolic and transcriptomic profiles of glioblastoma invasion revealed by comparisons between patients and corresponding orthotopic xenografts in mice. Acta Neuropathol Commun. 2021;9:133 pubmed publisher
Humeau J, Sauvat A, Cerrato G, Xie W, Loos F, Iannantuoni F, et al. Inhibition of transcription by dactinomycin reveals a new characteristic of immunogenic cell stress. EMBO Mol Med. 2020;12:e11622 pubmed publisher
Chesnelong C, Hao X, Cseh O, Wang A, Luchman H, Weiss S. SLUG Directs the Precursor State of Human Brain Tumor Stem Cells. Cancers (Basel). 2019;11: pubmed publisher
Bütepage M, Preisinger C, von Kriegsheim A, Scheufen A, Lausberg E, Li J, et al. Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription. Sci Rep. 2018;8:6748 pubmed publisher
Liang X, Nichols J, Sun H, Crooke S. Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs. Nucleic Acids Res. 2017;: pubmed publisher
Marcel V, Catez F, Berger C, Perrial E, Plesa A, Thomas X, et al. Expression Profiling of Ribosome Biogenesis Factors Reveals Nucleolin as a Novel Potential Marker to Predict Outcome in AML Patients. PLoS ONE. 2017;12:e0170160 pubmed publisher
Holmberg Olausson K, Nistér M, Lindström M. Loss of nucleolar histone chaperone NPM1 triggers rearrangement of heterochromatin and synergizes with a deficiency in DNA methyltransferase DNMT3A to drive ribosomal DNA transcription. J Biol Chem. 2014;289:34601-19 pubmed publisher
Guo X, Xiong L, Yu L, Li R, Wang Z, Ren B, et al. Increased level of nucleolin confers to aggressive tumor progression and poor prognosis in patients with hepatocellular carcinoma after hepatectomy. Diagn Pathol. 2014;9:175 pubmed publisher
Park R, El Guindy A, Heston L, Lin S, Yu K, Nagy M, et al. Nuclear translocation and regulation of intranuclear distribution of cytoplasmic poly(A)-binding protein are distinct processes mediated by two Epstein Barr virus proteins. PLoS ONE. 2014;9:e92593 pubmed publisher
Qiu J, Chen S, Su L, Liu J, Xiao N, Ou T, et al. Cellular nucleic acid binding protein suppresses tumor cell metastasis and induces tumor cell death by downregulating heterogeneous ribonucleoprotein K in fibrosarcoma cells. Biochim Biophys Acta. 2014;1840:2244-52 pubmed publisher
Liang X, Crooke S. RNA helicase A is not required for RISC activity. Biochim Biophys Acta. 2013;1829:1092-101 pubmed publisher
Simpson K, Cawthorne C, Zhou C, Hodgkinson C, Walker M, Trapani F, et al. A caspase-3 'death-switch' in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers. Cell Death Dis. 2013;4:e613 pubmed publisher
Chang T, Qi C, Meng J, Zhang N, Bing T, Yang X, et al. General cell-binding activity of intramolecular G-quadruplexes with parallel structure. PLoS ONE. 2013;8:e62348 pubmed publisher
Santoro R, Mori F, Marani M, Grasso G, Cambria M, Blandino G, et al. Blockage of melatonin receptors impairs p53-mediated prevention of DNA damage accumulation. Carcinogenesis. 2013;34:1051-61 pubmed publisher
Dutta B, Adav S, Koh C, Lim S, Meshorer E, Sze S. Elucidating the temporal dynamics of chromatin-associated protein release upon DNA digestion by quantitative proteomic approach. J Proteomics. 2012;75:5493-506 pubmed publisher
Sharma S, Burdon K, Chidlow G, Klebe S, Crawford A, Dimasi D, et al. Association of genetic variants in the TMCO1 gene with clinical parameters related to glaucoma and characterization of the protein in the eye. Invest Ophthalmol Vis Sci. 2012;53:4917-25 pubmed publisher
Cornelissen B, Waller A, Target C, Kersemans V, Smart S, Vallis K. 111In-BnDTPA-F3: an Auger electron-emitting radiotherapeutic agent that targets nucleolin. EJNMMI Res. 2012;2:9 pubmed publisher
Santoro R, Marani M, Blandino G, Muti P, Strano S. Melatonin triggers p53Ser phosphorylation and prevents DNA damage accumulation. Oncogene. 2012;31:2931-42 pubmed publisher
Koh C, Gurel B, Sutcliffe S, Aryee M, Schultz D, Iwata T, et al. Alterations in nucleolar structure and gene expression programs in prostatic neoplasia are driven by the MYC oncogene. Am J Pathol. 2011;178:1824-34 pubmed publisher
Park R, Wang ondu R, Heston L, Shedd D, Miller G. Efficient induction of nuclear aggresomes by specific single missense mutations in the DNA-binding domain of a viral AP-1 homolog. J Biol Chem. 2011;286:9748-62 pubmed publisher
Liang X, Vickers T, Guo S, Crooke S. Efficient and specific knockdown of small non-coding RNAs in mammalian cells and in mice. Nucleic Acids Res. 2011;39:e13 pubmed publisher
Munday D, Emmott E, Surtees R, Lardeau C, Wu W, Duprex W, et al. Quantitative proteomic analysis of A549 cells infected with human respiratory syncytial virus. Mol Cell Proteomics. 2010;9:2438-59 pubmed publisher
Peng L, Liang J, Wang H, Song X, Rashid A, Gomez H, et al. High levels of nucleolar expression of nucleolin are associated with better prognosis in patients with stage II pancreatic ductal adenocarcinoma. Clin Cancer Res. 2010;16:3734-42 pubmed publisher
Battaglia S, Maguire O, Thorne J, Hornung L, Doig C, Liu S, et al. Elevated NCOR1 disrupts PPARalpha/gamma signaling in prostate cancer and forms a targetable epigenetic lesion. Carcinogenesis. 2010;31:1650-60 pubmed publisher
Boyne J, Whitehouse A. Nucleolar disruption impairs Kaposi's sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs. FEBS Lett. 2009;583:3549-56 pubmed publisher
Sato H, Kusumoto Matsuo R, Ishii Y, Mori S, Nakahara T, Shinkai Ouchi F, et al. Identification of nucleolin as a protein that binds to human papillomavirus type 16 DNA. Biochem Biophys Res Commun. 2009;387:525-30 pubmed publisher
Kilday J, Rahman R, Dyer S, Ridley L, Lowe J, Coyle B, et al. Pediatric ependymoma: biological perspectives. Mol Cancer Res. 2009;7:765-86 pubmed publisher
Han Z, Alves C, Gudima S, Taylor J. Intracellular localization of hepatitis delta virus proteins in the presence and absence of viral RNA accumulation. J Virol. 2009;83:6457-63 pubmed publisher
Potapova T, Daum J, Byrd K, Gorbsky G. Fine tuning the cell cycle: activation of the Cdk1 inhibitory phosphorylation pathway during mitotic exit. Mol Biol Cell. 2009;20:1737-48 pubmed publisher
Abedin S, Thorne J, Battaglia S, Maguire O, Hornung L, Doherty A, et al. Elevated NCOR1 disrupts a network of dietary-sensing nuclear receptors in bladder cancer cells. Carcinogenesis. 2009;30:449-56 pubmed publisher
Ridley L, Rahman R, Brundler M, Ellison D, Lowe J, Robson K, et al. Multifactorial analysis of predictors of outcome in pediatric intracranial ependymoma. Neuro Oncol. 2008;10:675-89 pubmed publisher
Kranz D, Dohmesen C, Dobbelstein M. BRCA1 and Tip60 determine the cellular response to ultraviolet irradiation through distinct pathways. J Cell Biol. 2008;182:197-213 pubmed publisher
Yang L, Reece J, Cho J, Bortner C, Shears S. The nucleolus exhibits an osmotically regulated gatekeeping activity that controls the spatial dynamics and functions of nucleolin. J Biol Chem. 2008;283:11823-31 pubmed publisher
Sturchler E, Cox J, Durussel I, Weibel M, Heizmann C. S100A16, a novel calcium-binding protein of the EF-hand superfamily. J Biol Chem. 2006;281:38905-17 pubmed
product information
CatalogNumber :
ab13541
ItemName :
Anti-Nucleolin antibody [4E2] - ChIP Grade
Product Type :
Antibody
Host Species :
Mouse
Clonality :
Monoclonal
Conjugate :
Unconjugated
Clone Name :
4E2
Size :
50 µg
SpeciesSummary :
Hu
Purity :
Protein A purified
Application Summary :
ChIP, Flow Cyt, ICC, ICC/IF, IHC-P, WB
company information
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB20AX
https://www.abcam.com
888-7722226
headquarters: UK