This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Abcam
other brands :
Epitomics, Ascent Scientific, MitoSciences
product type :
antibody
product name :
Anti-5-methylcytosine (5-mC) antibody [33D3]
catalog :
ab10805
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
33D3
reactivity :
Entamoeba histolytica, human, mouse, rat, pigs
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section, dot blot, other
citations: 40
Published Application/Species/Sample/DilutionReference
  • immunoprecipitation; mouse; loading ...; fig 4f
Aibara D, Takahashi S, Yagai T, Kim D, Brocker C, Levi M, et al. Gene repression through epigenetic modulation by PPARA enhances hepatocellular proliferation. iScience. 2022;25:104196 pubmed publisher
  • chromatin immunoprecipitation; human; 1:100; loading ...; fig 5g
Wu S, Xiao Y, Wei J, Xu X, Jin X, Hu X, et al. MYC suppresses STING-dependent innate immunity by transcriptionally upregulating DNMT1 in triple-negative breast cancer. J Immunother Cancer. 2021;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1a
Moyon S, Frawley R, Maréchal D, Huang D, Marshall Phelps K, Kegel L, et al. TET1-mediated DNA hydroxymethylation regulates adult remyelination in mice. Nat Commun. 2021;12:3359 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 2c
Wang J, Zhu W, Han J, Yang X, Zhou R, Lu H, et al. The role of the HIF-1α/ALYREF/PKM2 axis in glycolysis and tumorigenesis of bladder cancer. Cancer Commun (Lond). 2021;41:560-575 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 3b
Zhu X, Wang X, Yan W, Yang H, Xiang Y, Lv F, et al. Ubiquitination-mediated degradation of TRDMT1 regulates homologous recombination and therapeutic response. NAR Cancer. 2021;3:zcab010 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 7a
Zheng Z, Zeng S, Liu C, Li W, Zhao L, Cai C, et al. The DNA methylation inhibitor RG108 protects against noise-induced hearing loss. Cell Biol Toxicol. 2021;37:751-771 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 2a
Wu T, Zhang Z, Li S, Wang B, Yang Z, Li P, et al. Characterization of global 5-hydroxymethylcytosine in pediatric posterior fossa ependymoma. Clin Epigenetics. 2020;12:19 pubmed publisher
  • western blot; human; loading ...; fig s3a
Mohni K, Wessel S, Zhao R, Wojciechowski A, Luzwick J, Layden H, et al. HMCES Maintains Genome Integrity by Shielding Abasic Sites in Single-Strand DNA. Cell. 2019;176:144-153.e13 pubmed publisher
  • other; mouse; fig 1c
Raciti G, Spinelli R, Desiderio A, Longo M, Parrillo L, Nigro C, et al. Specific CpG hyper-methylation leads to Ankrd26 gene down-regulation in white adipose tissue of a mouse model of diet-induced obesity. Sci Rep. 2017;7:43526 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 3a
Wang X, Wang L, Sun Y, Li R, Deng J, Deng J. DNA methylation and histone deacetylation regulating insulin sensitivity due to chronic cold exposure. Cryobiology. 2017;74:36-42 pubmed publisher
  • dot blot; human; loading ...; fig 1e
Zhou X, Zhuang Z, Wang W, He L, Wu H, Cao Y, et al. OGG1 is essential in oxidative stress induced DNA demethylation. Cell Signal. 2016;28:1163-71 pubmed publisher
  • immunoprecipitation; rat; fig 5
Su R, Yan J, Yang H. Transgenerational Glucose Intolerance of Tumor Necrosis Factor with Epigenetic Alteration in Rat Perirenal Adipose Tissue Induced by Intrauterine Hyperglycemia. J Diabetes Res. 2016;2016:4952801 pubmed publisher
  • immunocytochemistry; Entamoeba histolytica; 1:25; fig 3
Lozano Amado D, Herrera Solorio A, Valdés J, Alemán Lazarini L, Almaraz Barrera M, Luna Rivera E, et al. Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica. Parasit Vectors. 2016;9:19 pubmed publisher
  • immunohistochemistry - free floating section; pigs ; 1:200
Cao Z, Zhou N, Zhang Y, Zhang Y, Wu R, Li Y, et al. Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis. Theriogenology. 2014;81:496-508 pubmed publisher
  • chromatin immunoprecipitation; pigs ; 2 ug/time
Jia Y, Li R, Cong R, Yang X, Sun Q, Parvizi N, et al. Maternal low-protein diet affects epigenetic regulation of hepatic mitochondrial DNA transcription in a sex-specific manner in newborn piglets associated with GR binding to its promoter. PLoS ONE. 2013;8:e63855 pubmed publisher
  • immunoprecipitation; pigs ; 1 ug
Li R, Zou H, Jia Y, Zhao R. Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of mtDNA- and nuclear-encoded mitochondria genes in the liver of newborn piglets. BMC Vet Res. 2013;9:87 pubmed publisher
Irie N, Lee S, Lorenzi V, Xu H, Chen J, Inoue M, et al. DMRT1 regulates human germline commitment. Nat Cell Biol. 2023;25:1439-1452 pubmed publisher
Jiang B, Ding T, Guo C, Bai X, Cao D, Wu X, et al. NFAT1 Orchestrates Spinal Microglial Transcription and Promotes Microglial Proliferation via c-MYC Contributing to Nerve Injury-Induced Neuropathic Pain. Adv Sci (Weinh). 2022;9:e2201300 pubmed publisher
Zhong R, Zhang F, Yang Z, Li Y, Xu Q, Lan H, et al. Regulation of Ion Channel Function in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes by Cancer Cell Secretion Through DNA Methylation. Front Cardiovasc Med. 2022;9:839104 pubmed publisher
Zhang D, Ning J, Okon I, Zheng X, Satyanarayana G, Song P, et al. Suppression of m6A mRNA modification by DNA hypermethylated ALKBH5 aggravates the oncological behavior of KRAS mutation/LKB1 loss lung cancer. Cell Death Dis. 2021;12:518 pubmed publisher
Li M, Jiang P, Cheng K, Zhang Z, Lan S, Li X, et al. Regulation of MYB by distal enhancer elements in human myeloid leukemia. Cell Death Dis. 2021;12:223 pubmed publisher
Bettencourt C, Foti S, Miki Y, Botia J, Chatterjee A, Warner T, et al. White matter DNA methylation profiling reveals deregulation of HIP1, LMAN2, MOBP, and other loci in multiple system atrophy. Acta Neuropathol. 2020;139:135-156 pubmed publisher
Fillatre J, Fauny J, Fels J, Li C, Goll M, Thisse C, et al. TEADs, Yap, Taz, Vgll4s transcription factors control the establishment of Left-Right asymmetry in zebrafish. elife. 2019;8: pubmed publisher
de Souza A, Pieri N, Roballo K, Bressan F, Casals J, Ambrosio C, et al. Dynamics of male canine germ cell development. PLoS ONE. 2018;13:e0193026 pubmed publisher
Stefansson O, Hermanowicz S, van der Horst J, Hilmarsdottir H, Staszczak Z, Jonasson J, et al. CpG promoter methylation of the ALKBH3 alkylation repair gene in breast cancer. BMC Cancer. 2017;17:469 pubmed publisher
Ohta H, Kurimoto K, Okamoto I, Nakamura T, Yabuta Y, Miyauchi H, et al. In vitro expansion of mouse primordial germ cell-like cells recapitulates an epigenetic blank slate. EMBO J. 2017;36:1888-1907 pubmed publisher
Amankulor N, Kim Y, Arora S, Kargl J, Szulzewsky F, Hanke M, et al. Mutant IDH1 regulates the tumor-associated immune system in gliomas. Genes Dev. 2017;31:774-786 pubmed publisher
Jiang X, Feng Y, Ye L, Li X, Feng J, Zhang M, et al. Inhibition of Gata4 and Tbx5 by Nicotine-Mediated DNA Methylation in Myocardial Differentiation. Stem Cell Reports. 2017;8:290-304 pubmed publisher
Guo W, Chen J, Yang Y, Zhu J, Wu J. Epigenetic programming of Dnmt3a mediated by AP2α is required for granting preadipocyte the ability to differentiate. Cell Death Dis. 2016;7:e2496 pubmed publisher
Klasić M, Krištić J, Korac P, Horvat T, Markulin D, Vojta A, et al. DNA hypomethylation upregulates expression of the MGAT3 gene in HepG2 cells and leads to changes in N-glycosylation of secreted glycoproteins. Sci Rep. 2016;6:24363 pubmed publisher
Sun Q, Li X, Jia Y, Pan S, Li R, Yang X, et al. Maternal betaine supplementation during gestation modifies hippocampal expression of GR and its regulatory miRNAs in neonatal piglets. J Vet Med Sci. 2016;78:921-8 pubmed publisher
Hu Y, Sun Q, Li X, Wang M, Cai D, Li X, et al. In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks. PLoS ONE. 2015;10:e0122643 pubmed publisher
Irie N, Weinberger L, Tang W, Kobayashi T, Viukov S, Manor Y, et al. SOX17 is a critical specifier of human primordial germ cell fate. Cell. 2015;160:253-68 pubmed publisher
Farinelli P, Perera A, Arango Gonzalez B, Trifunovic D, Wagner M, Carell T, et al. DNA methylation and differential gene regulation in photoreceptor cell death. Cell Death Dis. 2014;5:e1558 pubmed publisher
Cai D, Jia Y, Song H, Sui S, Lu J, Jiang Z, et al. Betaine supplementation in maternal diet modulates the epigenetic regulation of hepatic gluconeogenic genes in neonatal piglets. PLoS ONE. 2014;9:e105504 pubmed publisher
Bočkor V, Barišić D, Horvat T, Maglica Z, Vojta A, Zoldos V. Inhibition of DNA methylation alters chromatin organization, nuclear positioning and activity of 45S rDNA loci in cycling cells of Q. robur. PLoS ONE. 2014;9:e103954 pubmed publisher
Nettersheim D, Heukamp L, Fronhoffs F, Grewe M, Haas N, Waha A, et al. Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development. PLoS ONE. 2013;8:e82881 pubmed publisher
Mould A, Pang Z, Pakusch M, Tonks I, Stark M, Carrie D, et al. Smchd1 regulates a subset of autosomal genes subject to monoallelic expression in addition to being critical for X inactivation. Epigenetics Chromatin. 2013;6:19 pubmed publisher
Lee J, Jeong W, Lim W, Lim C, Bae S, Kim J, et al. Hypermethylation and post-transcriptional regulation of DNA methyltransferases in the ovarian carcinomas of the laying hen. PLoS ONE. 2013;8:e61658 pubmed publisher
Bracht J, Perlman D, Landweber L. Cytosine methylation and hydroxymethylation mark DNA for elimination in Oxytricha trifallax. Genome Biol. 2012;13:R99 pubmed publisher
product information
CatalogNumber :
ab10805
ItemName :
Anti-5-methylcytosine (5-mC) antibody [33D3]
Category :
Antibody
Host :
Mouse
Clonality :
Monoclonal
Conjugate :
Unconjugated
Clone Name :
33D3
Size :
50 µg
Application Summary :
ChIP, Dot, Flow Cyt, IHC-Fr, IHC-P, IP, MeDIP, SB
company information
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB20AX
Stefan.Pritchard@abcam.com, eCommerce@abcam.com, Vicky.Yang@abcam.com
https://www.abcam.com
888-7722226
headquarters: UK