This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Abcam
other brands :
Epitomics, Ascent Scientific, MitoSciences
product type :
antibody
product name :
Anti-GFAP antibody [GF5]
catalog :
ab10062
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GF5
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 53
Published Application/Species/Sample/DilutionReference
  • flow cytometry; rat; loading ...; fig 4c
Chen Y, Li J, Ma B, Li N, Wang S, Sun Z, et al. MSC-derived exosomes promote recovery from traumatic brain injury via microglia/macrophages in rat. Aging (Albany NY). 2020;12:18274-18296 pubmed publisher
  • immunohistochemistry; rat; 1:1000; loading ...; fig 3d
Li R, Li D, Wu C, Ye L, Wu Y, Yuan Y, et al. Nerve growth factor activates autophagy in Schwann cells to enhance myelin debris clearance and to expedite nerve regeneration. Theranostics. 2020;10:1649-1677 pubmed publisher
  • immunocytochemistry; mouse; 1:300; loading ...; fig 1b
  • immunohistochemistry; mouse; 1:300; loading ...; fig 2b
Zhu Q, Zhang N, Hu N, Jiang R, Lu H, Xuan A, et al. Neural stem cell transplantation improves learning and memory by protecting cholinergic neurons and restoring synaptic impairment in an amyloid precursor protein/presenilin 1 transgenic mouse model of Alzheimer's disease. Mol Med Rep. 2020;21:1172-1180 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 1b
Murlidharan G, Sakamoto K, Rao L, Corriher T, Wang D, Gao G, et al. CNS-restricted Transduction and CRISPR/Cas9-mediated Gene Deletion with an Engineered AAV Vector. Mol Ther Nucleic Acids. 2016;5:e338 pubmed publisher
  • immunohistochemistry - paraffin section; rat; fig 1
Zhai W, Chen D, Shen H, Chen Z, Li H, Yu Z, et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain. 2016;9:66 pubmed publisher
  • immunohistochemistry; human; 1:100; fig 2
Janmaat C, de Rooij K, Locher H, de Groot S, de Groot J, Frijns J, et al. Human Dermal Fibroblasts Demonstrate Positive Immunostaining for Neuron- and Glia- Specific Proteins. PLoS ONE. 2015;10:e0145235 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:100; fig 3
Gu Y, Zhang Y, Bi Y, Liu J, Tan B, Gong M, et al. Mesenchymal stem cells suppress neuronal apoptosis and decrease IL-10 release via the TLR2/NFκB pathway in rats with hypoxic-ischemic brain damage. Mol Brain. 2015;8:65 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 2
  • immunocytochemistry; mouse; fig 4
Chen H, Sun Y, Lai L, Wu H, Xiao Y, Ming B, et al. Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice. Neuroscience. 2015;308:157-68 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2a
Huang Y, Tiao M, Huang L, Chuang J, Kuo K, Yang Y, et al. Activation of Mir-29a in Activated Hepatic Stellate Cells Modulates Its Profibrogenic Phenotype through Inhibition of Histone Deacetylases 4. PLoS ONE. 2015;10:e0136453 pubmed publisher
  • western blot; mouse; 1:1000; fig 1
Korb E, Herre M, Zucker Scharff I, Darnell R, Allis C. BET protein Brd4 activates transcription in neurons and BET inhibitor Jq1 blocks memory in mice. Nat Neurosci. 2015;18:1464-73 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig s2a
Frank C, Liu F, Wijayatunge R, Song L, Biegler M, Yang M, et al. Regulation of chromatin accessibility and Zic binding at enhancers in the developing cerebellum. Nat Neurosci. 2015;18:647-56 pubmed publisher
  • immunohistochemistry - free floating section; rat; 1:500
  • immunohistochemistry; rat; 1:500
  • western blot; rat; 1:500
Filipcik P, Cente M, Zilka N, Smolek T, Hanes J, Kučerák J, et al. Intraneuronal accumulation of misfolded tau protein induces overexpression of Hsp27 in activated astrocytes. Biochim Biophys Acta. 2015;1852:1219-29 pubmed publisher
  • immunohistochemistry; mouse; 1:250; fig 5
Porquet D, Andrés Benito P, Griñán Ferré C, Camins A, Ferrer I, Canudas A, et al. Amyloid and tau pathology of familial Alzheimer's disease APP/PS1 mouse model in a senescence phenotype background (SAMP8). Age (Dordr). 2015;37:9747 pubmed publisher
  • immunocytochemistry; human; 1:100; fig 1
Spilsbury A, Miwa S, Attems J, Saretzki G. The role of telomerase protein TERT in Alzheimer's disease and in tau-related pathology in vitro. J Neurosci. 2015;35:1659-74 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Wijayatunge R, Chen L, Cha Y, Zannas A, Frank C, West A. The histone lysine demethylase Kdm6b is required for activity-dependent preconditioning of hippocampal neuronal survival. Mol Cell Neurosci. 2014;61:187-200 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:250
Merres J, Höss J, Albrecht L, Kress E, Soehnlein O, Jansen S, et al. Role of the cathelicidin-related antimicrobial peptide in inflammation and mortality in a mouse model of bacterial meningitis. J Innate Immun. 2014;6:205-18 pubmed publisher
  • immunohistochemistry - frozen section; rat; 1:500
  • western blot; rat; 1:500
Sahu S, Kauser H, Ray K, Kishore K, Kumar S, Panjwani U. Caffeine and modafinil promote adult neuronal cell proliferation during 48 h of total sleep deprivation in rat dentate gyrus. Exp Neurol. 2013;248:470-81 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:250
Slowik A, Merres J, Elfgen A, Jansen S, Mohr F, Wruck C, et al. Involvement of formyl peptide receptors in receptor for advanced glycation end products (RAGE)--and amyloid beta 1-42-induced signal transduction in glial cells. Mol Neurodegener. 2012;7:55 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:250
Brandenburg L, Jansen S, Albrecht L, Merres J, Gerber J, Pufe T, et al. CpG oligodeoxynucleotides induce the expression of the antimicrobial peptide cathelicidin in glial cells. J Neuroimmunol. 2013;255:18-31 pubmed publisher
Wan B, Li C, Wang M, Kong F, Ding Q, Zhang C, et al. GIT1 protects traumatically injured spinal cord by prompting microvascular endothelial cells to clear myelin debris. Aging (Albany NY). 2021;13:7067-7083 pubmed publisher
Elsherbini A, Kirov A, Dinkins M, Wang G, Qin H, Zhu Z, et al. Association of Aβ with ceramide-enriched astrosomes mediates Aβ neurotoxicity. Acta Neuropathol Commun. 2020;8:60 pubmed publisher
Tan Z, Chen L, Ren Y, Jiang X, Gao W. Neuroprotective effects of FK866 against traumatic brain injury: Involvement of p38/ERK pathway. Ann Clin Transl Neurol. 2020;7:742-756 pubmed publisher
Liu W, Han F, Qu S, Yao Y, Zhao J, Akhtar M, et al. MARVELD1 depletion leads to dysfunction of motor and cognition via regulating glia-dependent neuronal migration during brain development. Cell Death Dis. 2018;9:999 pubmed publisher
Xiao K, Sun Z, Jin X, Ma W, Song Y, Lai S, et al. ERG3 potassium channel-mediated suppression of neuronal intrinsic excitability and prevention of seizure generation in mice. J Physiol. 2018;596:4729-4752 pubmed publisher
Panikker P, Xu S, Zhang H, Sarthi J, Beaver M, Sheth A, et al. Restoring Tip60 HAT/HDAC2 Balance in the Neurodegenerative Brain Relieves Epigenetic Transcriptional Repression and Reinstates Cognition. J Neurosci. 2018;38:4569-4583 pubmed publisher
Tan V, Mockett B, Ohline S, Parfitt K, Wicky H, Peppercorn K, et al. Lentivirus-mediated expression of human secreted amyloid precursor protein-alpha prevents development of memory and plasticity deficits in a mouse model of Alzheimer's disease. Mol Brain. 2018;11:7 pubmed publisher
Pan Z, Shan Q, Gu P, Wang X, Tai L, Sun M, et al. miRNA-23a/CXCR4 regulates neuropathic pain via directly targeting TXNIP/NLRP3 inflammasome axis. J Neuroinflammation. 2018;15:29 pubmed publisher
Li Z, Li Y, Cao J, Han X, Cai W, Zang W, et al. Membrane protein Nav1.7 contributes to the persistent post-surgical pain regulated by p-p65 in dorsal root ganglion (DRG) of SMIR rats model. BMC Anesthesiol. 2017;17:150 pubmed publisher
Jongbloets B, Lemstra S, Schellino R, Broekhoven M, Parkash J, Hellemons A, et al. Stage-specific functions of Semaphorin7A during adult hippocampal neurogenesis rely on distinct receptors. Nat Commun. 2017;8:14666 pubmed publisher
Shen Y, Qin H, Chen J, Mou L, He Y, Yan Y, et al. Postnatal activation of TLR4 in astrocytes promotes excitatory synaptogenesis in hippocampal neurons. J Cell Biol. 2016;215:719-734 pubmed
Tang W, Hsu Y, Wang C, Hu C, Chio C, Kuo J. Early electroacupuncture treatment ameliorates neuroinflammation in rats with traumatic brain injury. BMC Complement Altern Med. 2016;16:470 pubmed
Sun T, Chen G, Li Y, Xie X, Zhou Y, Du Z. Aggressive invasion is observed in CD133-/A2B5+ glioma-initiating cells. Oncol Lett. 2015;10:3399-3406 pubmed
Wang Y, Wang D, Guo D. MiR-124 Promote Neurogenic Transdifferentiation of Adipose Derived Mesenchymal Stromal Cells Partly through RhoA/ROCK1, but Not ROCK2 Signaling Pathway. PLoS ONE. 2016;11:e0146646 pubmed publisher
Dehghan S, Asadi S, Hajikaram M, Soleimani M, Mowla S, Fathollahi Y, et al. Exogenous Oct4 in combination with valproic acid increased neural progenitor markers: an approach for enhancing the repair potential of the brain. Life Sci. 2015;122:108-15 pubmed publisher
Ding D, Xu L, Xu H, Li X, Liang Q, Zhao Y, et al. Mash1 efficiently reprograms rat astrocytes into neurons. Neural Regen Res. 2014;9:25-32 pubmed publisher
Li H, Wang Y, Feng D, Liu Y, Xu M, Gao A, et al. Alterations in the time course of expression of the Nox family in the brain in a rat experimental cerebral ischemia and reperfusion model: effects of melatonin. J Pineal Res. 2014;57:110-9 pubmed publisher
Ando K, Brion J, Stygelbout V, Suain V, Authelet M, Dedecker R, et al. Clathrin adaptor CALM/PICALM is associated with neurofibrillary tangles and is cleaved in Alzheimer's brains. Acta Neuropathol. 2013;125:861-78 pubmed publisher
Jansen S, Podschun R, Leib S, Grötzinger J, Oestern S, Michalek M, et al. Expression and function of psoriasin (S100A7) and koebnerisin (S100A15) in the brain. Infect Immun. 2013;81:1788-97 pubmed publisher
Huang J, Shang L, Zhang M, Wang H, Chen D, Tong J, et al. Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response. BMC Neurosci. 2013;14:16 pubmed publisher
Wang X, Ma A, Zhu W, Zhu L, Zhao Y, Xi J, et al. The role of connexin 43 and hemichannels correlated with the astrocytic death following ischemia/reperfusion insult. Cell Mol Neurobiol. 2013;33:401-10 pubmed publisher
Zhao X, Zhang H, Guo J, Zhang Y. The effects of bilateral common carotid artery occlusion on expression of peripherin and choline acetyltransferase activity in C57BL/6 mice. Brain Res. 2013;1491:167-75 pubmed publisher
Lebonvallet N, Boulais N, Le Gall C, Chéret J, Pereira U, Mignen O, et al. Characterization of neurons from adult human skin-derived precursors in serum-free medium : a PCR array and immunocytological analysis. Exp Dermatol. 2012;21:195-200 pubmed publisher
Shah A, Singh D, Buch S, Kumar A. HIV-1 envelope protein gp120 up regulates CCL5 production in astrocytes which can be circumvented by inhibitors of NF-?B pathway. Biochem Biophys Res Commun. 2011;414:112-7 pubmed publisher
Schott B, Frischknecht R, Debska Vielhaber G, John N, Behnisch G, Duzel E, et al. Membrane-Bound Catechol-O-Methyl Transferase in Cortical Neurons and Glial Cells is Intracellularly Oriented. Front Psychiatry. 2010;1:142 pubmed publisher
Braun B, Slowik A, Leib S, Lucius R, Varoga D, Wruck C, et al. The formyl peptide receptor like-1 and scavenger receptor MARCO are involved in glial cell activation in bacterial meningitis. J Neuroinflammation. 2011;8:11 pubmed publisher
Kuang X, Zhao X, Xu H, Shi Y, Deng J, Sun G. Spatio-temporal expression of a novel neuron-derived neurotrophic factor (NDNF) in mouse brains during development. BMC Neurosci. 2010;11:137 pubmed publisher
Rump T, Abdul Muneer P, Szlachetka A, Lamb A, Haorei C, Alikunju S, et al. Acetyl-L-carnitine protects neuronal function from alcohol-induced oxidative damage in the brain. Free Radic Biol Med. 2010;49:1494-504 pubmed publisher
Clarner T, Buschmann J, Beyer C, Kipp M. Glial amyloid precursor protein expression is restricted to astrocytes in an experimental toxic model of multiple sclerosis. J Mol Neurosci. 2011;43:268-74 pubmed publisher
O Sullivan N, Pickering M, Di Giacomo D, Loscher J, Murphy K. Mkl transcription cofactors regulate structural plasticity in hippocampal neurons. Cereb Cortex. 2010;20:1915-25 pubmed publisher
Chandrasekar V, Dreyer J. The brain-specific Neural Zinc Finger transcription factor 2b (NZF-2b/7ZFMyt1) causes suppression of cocaine-induced locomotor activity. Neurobiol Dis. 2010;37:86-98 pubmed publisher
da Silveira A, Freitas M, de Oliveira E, Neto S, Luquetti A, Furness J, et al. Glial fibrillary acidic protein and S-100 colocalization in the enteroglial cells in dilated and nondilated portions of colon from chagasic patients. Hum Pathol. 2009;40:244-51 pubmed publisher
Moser J, Eystathioy T, Chan E, Fritzler M. Markers of mRNA stabilization and degradation, and RNAi within astrocytoma GW bodies. J Neurosci Res. 2007;85:3619-31 pubmed
Clark A, D Aquisto F, Gentry C, Marchand F, McMahon S, Malcangio M. Rapid co-release of interleukin 1beta and caspase 1 in spinal cord inflammation. J Neurochem. 2006;99:868-80 pubmed
product information
CatalogNumber :
ab10062
ItemName :
Anti-GFAP antibody [GF5]
Product Type :
Antibody
Host Species :
Mouse
Clonality :
Monoclonal
Conjugate :
Unconjugated
Clone Name :
GF5
Size :
100 µg
SpeciesSummary :
Hu, Ms, Rat
Purity :
Protein G purified
Application Summary :
ELISA, Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
company information
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB20AX
https://www.abcam.com
888-7722226
headquarters: UK