GAPDH (1A10) Antibody | Abbiotec 252626 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Abbiotec

product type :

antibody

product name :

GAPDH (1A10) Antibody

catalog :

252626

quantity :

0.1 ml

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

citations: 1

Reference
Helsby M, Leader P, Fenn J, Gulsen T, Bryant C, Doughton G, et al. CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited. BMC Cell Biol. 2014;15:6 pubmed publisher

product information

Product Name :

GAPDH (1A10) Antibody

Product Subcategory :

Mouse Monoclonal Antibody

Catalog No :

252626

Unit :

0.1 ml

Product Category :

Antibodies

Protein Family :

Enzymes

Pathway and Disease :

Carbohydrate Metabolism, Neurodegenerative Disorders

Description :

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair.

Format :

Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.

Applications :

E, WB, IHC, IF

Data :

20035WB.jpg; 5079IHC.jpg; 5079ICC.jpg

Data Legend :

The GAPDH (1A10) Antibody (Cat. No. 252626) is used in Western blot to detect GAPDH in human cell lysates. Lanes 1-9: HeLa, A549, A431, MCF-7, K562, Jurkat, HL60, SKN-SH, SKBR-3.; GAPDH staining in human breast and kidney carcinoma. Paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right) tissues are stained with GAPDH (1A10) Antibody (Cat. No. 252626) used at 1:200 dilution.; GAPDH staining in HepG2 and HeLa cells. Methanol-fixed HepG2 (left) and HeLa (right) human cells are stained with GAPDH (1A10) Antibody (Cat. No. 252626) used at 1:200 dilution (FITC and DRAQ5 staining in confocal microscopy).

Alternate Names :

Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH; GAPD; GAPDH

Accession No :

P04406

Clonality :

1A10

Isotype :

Mouse IgG1

Antigen :

Purified recombinant fragment of human GAPDH expressed in E. coli.

Species Reactivity :

Application Notes :

E: 1:10000; WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:200-1:1000

Storage :

Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment.