Human Histone H3.3 (H3F3A) ELISA Kit | Abbexa abx520521 product information

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product summary

company name :

Abbexa

product type :

ELISA/assay

product name :

Human Histone H3.3 (H3F3A) ELISA Kit

catalog :

abx520521

quantity :

96 tests

product information

Catalog Number :

abx520521

Target :

Histone H3.3

Product Name :

Human Histone H3.3 (H3F3A) ELISA Kit

Short Name :

H3F3A

Product Type :

ELISA Kits

Size 1 :

96 tests

Product Description :

Human Histone H3.3 (H3F3A) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Histone H3.3 concentrations in tissue homogenates, cell lysates and other biological fluids.

UniProt Primary AC :

P84243

UniProt Entry Name :

H33_HUMAN

Gene Symbol :

H3F3A

KEGG :

hsa:3020, hsa:3021

String :

9606.ENSP00000355778

CrossReactivity :

Human

Tested Applications :

ELISA

Storage :

Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

UNSPSC Code :

41116126

Test Range :

0.312 ng/ml - 20 ng/ml

Detection Method :

Colorimetric

Assay Type :

Sandwich

Assay Data :

Quantitative

Sample Type :

Tissue homogenates, cell lysates and other biological fluids.

Kit Components :

The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. Pre-coated 96-Well Microplate Standard Standard Diluent Buffer Wash Buffer Detection Reagent A Detection Reagent B Diluent A Diluent B TMB Substrate Stop Solution Plate Sealer

Material Required But Not Provided :

37°C incubator Multi and single channel pipettes and sterile pipette tips Squirt bottle or automated microplate washer 1.5 ml tubes Distilled water Absorbent filter papers 100 ml and 1 liter graduated cylinders Microplate reader (wavelength: 450 nm) ELISA Shaker

Protocol :

This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. Equilibrate the kit components and samples to room temperature (18 - 25 °C) before use. It is recommended to plot a standard curve for each test. 1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample at least in duplicate. 2. Add 100 µL of each standard, control and sample into the appropriate wells. Seal the plate with a cover and incubate for 1 h at 37°C. 3. Remove the cover and discard the liquid. 4. Add 100 µl of the detection Reagent A working solution to each well. Seal the plate with a cover and incubate for 1 h at 37°C. 5. Remove the cover and discard the solution. Wash the plate 3 times with 1X Wash Buffer. 6. Add 100 µL of Detection Reagent B working solution into each well, seal and incubate at 37°C for 30 min. 7. Discard the solution and wash the plate 5 times with wash buffer as explained in previous step. 8. Aliquot 90 µl of TMB Substrate into each well. Seal the plate with a cover and incubate at 37°C for 10-20 min. Avoid exposure to light. The incubation time is for reference use only, the optimal time should be determined by end user. Do not exceed 30 min. 9. Add 50 µL of Stop Solution to each well. Read at 450 nm immediately.

Availability :

Shipped within 5-12 working days.

Note :

This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.

Dry Ice :