Human PCNA antibody | NKMAX APC0828 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PCNA antibody

catalog :

APC0828

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

k1A3

reactivity :

human

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PCNA antibody

Catalog number (SKU) :

APC0828

NCBI :

NP_002583

Description :

PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighboring tumors.

Alternative Names :

Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Moldovan GL, et al., (2007) Cell 18 129(4):665-679.;; Matsumoto K , et al., (1987) EMBO 6(3):637-642.

Abs Host :

Mouse

Isotype :

IgG3 kappa

Immunogen :

Recombinant human PCNA (1-261aa) purified from E. coli

Applications :

ELISA,WB

WB Comment and Pic :

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCNA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HeLa cell lysate;;Lane 2.: NIH3T3 cell lysate

Reacts With :

Human

Clone Number :

k1A3

Ab Usage :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

Human

Gene Category :

Cell Cycle

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-G affinity chromatography

Product Type Name :

Monoclonal Antibody

UniProt No. :

P12004

Internal Search Terms :

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more info or order :

NKMAX product webpage