This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
ACROBiosystems
product type :
protein
product name :
Human Furin / PCSK3 Protein, His Tag
catalog :
FUN-H5221
quantity :
1 mg, 20 ug, 250 ug
price :
2500 USD, 300 USD, 980 USD
product information
company name :
ACROBiosystems
product type :
Protein
catalog :
FUN-H5221
product name :
Human Furin / PCSK3 Protein, His Tag
quantity :
1 mg, 20 ug, 250 ug
price :
2500 USD, 300 USD, 980 USD
quantity & price :
$300/20ug,$980/250ug,$2500/1mg (250ug × 4)
target :
Furin
host species :
Human
By Tag :
His Tag
Research :
For Research Use Only
Source :
Human Furin, His Tag (FUN-H5221) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gly 577 (Accession # AAH12181.1).
Endotoxin :
Less than 1.0 EU per μg by the LAL method.
Purity :
>95% as determined by SDS-PAGE.
SDS-PAGE Remark(R&NR) :
Human Furin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Formulation :
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, 1 mM CaCl2, pH9.0. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
Reconstitution :
Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage :
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at:
● -20°C to -70°C for 12 months in lyophilized state;
● -70℃ for 3 months under sterile conditions after reconstitution.
Background :
Furin is also known as paired basic Amino acid Cleaving Enzyme (PACE), is an enzyme which belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. The expression of furin in T-cells is required for maintenance of peripheral immune tolerance. Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg'). PACE is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. In addition to processing cellular precursor proteins, furin is also utilized by a number of pathogens. For example, the envelope proteins of viruses such as HIV, influenza and dengue fever viruses must be cleaved by furin or furin-like proteases to become fully functional. PACE also play a role in tumor progression.
References :
(1) Wise RJ., et al., 1991, Proc Natl Acad Sci U S A 87 (23): 9378–82.
(2) Kiefer MC., et al., 1992, DNA Cell Biol 10 (10): 757–69.
(3) Hallenberger S., et al., 1992, Nature 360 (6402): 358–61.
(4) Thomas G., 2002, Nat. Rev. Mol. Cell Biol. 3 (10): 753–66.
(5) Pesu M., et al., 2008, Nature 455 (7210): 246–50.
company information
ACROBiosystems
ACROBiosystems is a leading manufacturer of recombinant proteins and other critical reagents to support the development of target therapeutics. The company employs an application oriented development strategy, with a particular focus on product design, quality control and solution based support. Our products and services enable anyone in the field of drug development to have a more intuitive and streamlined process. We have multiple offices and branches in North America, Europe, and Asia, and we are proud of serving customers from over 50 countries.
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