This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
ACROBiosystems
product type :
protein
product name :
Human MCAM / CD146 Protein, Fc Tag
catalog :
CD6-H5253
quantity :
100 ug, 1 mg
price :
260 USD, 1800 USD
product information
company name :
ACROBiosystems
product type :
Protein
catalog :
CD6-H5253
product name :
Human MCAM / CD146 Protein, Fc Tag
quantity :
100 ug, 1 mg
price :
260 USD, 1800 USD
quantity & price :
$260/100ug,$1800/1mg
target :
MCAM
host species :
Human
By Tag :
Fc Tag
Research :
For Research Use Only
Source :
Human MCAM, Fc Tag (CD6-H5253) is expressed from human 293 cells (HEK293). It contains AA Val 24 - Gly 559 (Accession # P43121-1).
Endotoxin :
Less than 1.0 EU per μg by the LAL method.
Purity :
>95% as determined by SDS-PAGE.
SDS-PAGE Remark(R&NR) :
Human MCAM, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Formulation :
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
Reconstitution :
Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage :
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at:
● -20°C to -70°C for 12 months in lyophilized state;
● -70℃ for 3 months under sterile conditions after reconstitution.
Background :
CD146 is also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. As a member of the Immunoglobulin superfamily, It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.
CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells.
As a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor,CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis or immune recognition.
References :
(1) Kuske MD, et al.,1999, Cytogenet. Cell Genet. 87 (3-4): 258.
(2) Elshal MF, et al., 2005, Blood. 106 (8): 2923–4.
(3) Elshal MF, et al., 2007, BMC Immunol. 8: 29.
(4) Covas DT, et al., 2008, Exp. Hematol. 36 (5): 642–54.
(5) Russell KC, et al., 2010, Stem Cells 28 (4): 788–98.
company information
ACROBiosystems
ACROBiosystems is a leading manufacturer of recombinant proteins and other critical reagents to support the development of target therapeutics. The company employs an application oriented development strategy, with a particular focus on product design, quality control and solution based support. Our products and services enable anyone in the field of drug development to have a more intuitive and streamlined process. We have multiple offices and branches in North America, Europe, and Asia, and we are proud of serving customers from over 50 countries.
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