Human Carbonic Anhydrase II / CA2 Protein, His Tag | ACROBiosystems CA2-H5123 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

ACROBiosystems

product type :

protein

product name :

Human Carbonic Anhydrase II / CA2 Protein, His Tag

catalog :

CA2-H5123

quantity :

100 ug, 1 mg

price :

198 USD, 1200 USD

product information

company name :

ACROBiosystems

product type :

Protein

catalog :

CA2-H5123

product name :

Human Carbonic Anhydrase II / CA2 Protein, His Tag

quantity :

100 ug, 1 mg

price :

198 USD, 1200 USD

quantity & price :

$198/100ug,$1200/1mg (500ug × 2)

target :

Carbonic Anhydrase II

host species :

Human

By Tag :

His Tag

Research :

For Research Use Only

Source :

Human Carbonic Anhydrase II, His Tag (CA2-H5123) is expressed from E.coli cells. It contains AA Ser 2 - Lys 260 (Accession # P00918-1).

Endotoxin :

Less than 1.0 EU per μg by the LAL method.

Purity :

>95% as determined by SDS-PAGE.

SDS-PAGE Remark(R&NR) :

Human Carbonic Anhydrase II, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Formulation :

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.

Reconstitution :

Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage :

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: ● -20°C to -70°C for 12 months in lyophilized state; ● -70℃ for 3 months under sterile conditions after reconstitution.

Background :

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. They are, therefore, classified as metalloenzymes. There are at least five distinct CA families (α, β, γ, δ and ε). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The α-CAs are found in humans. Carbonic anhydrase II (CA2) is also known as Carbonate dehydratase II, Carbonic anhydrase C, is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

References :

(1) Badger MR, Price GD, 1994, Annu. Rev. Plant Physiol. Plant Mol. Bio. 45: 369–392. (2) Nair, S. K. et al., 1991, The Journal of biological chemistry 266 (26): 17320–17325. (3) Sterling, D., et al., 2001, J. Biol. Chem. (United States) 276 (51): 47886–94. (4) Vince, J W., et al., 2000, Biochemistry (UNITED STATES) 39 (44): 13344–9. (5) Li, Xiuju, et al., 2002, J. Biol. Chem. (United States) 277 (39): 36085–91.