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- mouse monoclonal (9-26)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of 14-3-3 alpha+beta on different lysates with Mouse anti-14-3-3 alpha+beta antibody (M0407-14) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-14-3-3 alpha+beta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0407-14) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of 14-3-3 alpha+beta on A549 cell lysates with Mouse anti-14-3-3 alpha+beta antibody (M0407-14) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0407-14) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis on mouse liver tissue lysates using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).
quantity: 100μl
price: 360.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - frozen section - domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section
gene information - zebrafish ywhabb
- synonym:wu:fb22g04; wu:fr86a12; ywhab; ywhab2; zgc:64092
- description:tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide b
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- ywhabb antibody
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