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- domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: A431 cell lysate Lane 4: Raji cell lysate Lane 5: HepG2 cell lysate Lane 6: SW480 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-1) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: Mouse skeletal muscle tissue lysate (40 µg/Lane) Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-1) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of VDAC1 on different lysates with Rabbit anti-VDAC1 antibody (R1307-1) at 1/1,000 dilution. Lane 1: HEK293-si NT cell lysate (10 µg/Lane) Lane 2: HEK293-si VDAC1#1(no heat) cell lysate (10 µg/Lane) Lane 3: HEK293-si VDAC1#2(no heat) cell lysate (10 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. R1307-1 was shown to specifically react with VDAC1 in HEK293-si NT cells. No band were observed when HEK293-si VDAC1 samples were tested. HEK293-si NT and HEK293-si VDAC1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (R1307-1, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
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gene information - zebrafish vdac1
- synonym:fa13f11; wu:fa13f11; zgc:85830
- description:voltage-dependent anion channel 1
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- vdac1 antibody
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