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- domestic rabbit monoclonal (JU30-49)
reactivity: human, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of USP14 on different lysates with Rabbit anti-USP14 antibody (ET1706-37) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si USP14 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of USP14 on different lysates with Rabbit anti-USP14 antibody (ET1706-37) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-37) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature.
Western blot analysis of USP14 on zebrafish lysates with Rabbit anti-USP14 antibody (ET1706-37) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-37) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
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gene information - zebrafish usp14
- synonym:wu:fk63d09; zgc:55949
- description:ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
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- usp14 antibody
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