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- domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (ER130905) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: A431 cell lysate (20 µg/Lane) Lane 7: K-562 cell lysate (20 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130905) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (ER130905) at different dilutions. Lane 1/2: NIH3T3 cell lysate at 1/1,000 and 1/5,000 dilution Lane 3/4: HepG2 cell lysate at 1/1,000 and 1/5,000 dilution Lane 5/6: PC-12 cell lysate at 1/1,000 and 1/5,000 dilution Lane 7/8: Hela cell lysate at 1/1,000 and 1/5,000 dilution Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130905) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
ICC staining of alpha Tubulin in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER130905, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section 
Dotblot: 1.Lac-tub primary antibody, 1:10,000 dilution (HA500554). 2. Exposure time: 60 s 3. 0.45 μm NC membrane
WB: 1.Lac-tub primary antibody, 1:2,000 dilution (HA500554). 2. HEK293T cell lysate from cells that were transfected with HA-tagged α-tubulinWT or α-tubulinK40A plasmids. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 s;10% SDS-PAGE gel. Proteins were transferred to a 0.45 μm NC membrane and blocked with 5% milk without fat in TBST for 0.5 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% BSA in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:10,000 dilution was used for 1 hour at room temperature.
WB: 1.Lac-tub primary antibody, 1:2,000 dilution (HA500554). 2. HEK293T cell lysate from cells that were treated with sodium lactate. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 s;10% SDS-PAGE gel.Proteins were transferred to a NC membrane and blocked with 5% milk without fat in TBST for 0.5 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% BSA in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:10,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier - mouse monoclonal (A8-6)
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of alpha Tubulin on different lysates with Mouse anti-alpha Tubulin antibody (M1501-1) at 1/10,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minute 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1501-1) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling alpha Tubulin with Mouse anti-alpha Tubulin antibody (M1501-1) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-alpha Tubulin antibody (M1501-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Immunocytochemistry analysis of U-87 MG cells labeling alpha Tubulin with Mouse anti-alpha Tubulin antibody (M1501-1) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-alpha Tubulin antibody (M1501-1) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 360.00 USD
to the supplier
gene information - zebrafish tuba8l3
- synonym:tuba8l3a; zgc:101117
- description:tubulin, alpha 8 like 3
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- tuba8l3 antibody
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