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- domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken, bovine, zebrafish
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay
Western blot analysis of SP1 (pT453) expression in Jurkat (A), mouse melanoma cancer (B) whole cell lysates.
Immunohistochemical analysis of SP1 (pT453) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of SP1 (pT453) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price: 165 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken, bovine, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry 
Western blot analysis of SMAD2 expression in SHSY5Y (A), K562 (B), COLO205 (C) whole cell lysates.
Immunohistochemical analysis of SMAD2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of SMAD2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price: 155 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, chicken, bovine, zebrafish
application: western blot, immunohistochemistry 
Western blot analysis of SMAD2 (pS465) expression in HepG2 (A) whole cell lysates.
Immunohistochemical analysis of SMAD2 (pS465) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
quantity: 0.03 mL
price: 165 USD
to the supplier - domestic rabbit monoclonal (A5S)
reactivity: human, mouse, rat, dogs, zebrafish
application: western blot, immunohistochemistry - paraffin section
citations: 14
gene information - zebrafish smad2
- synonym:fj43c06; madh2; wu:fj43c06
- description:SMAD family member 2
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- smad2 antibody
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