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- domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Histone H2B on different lysates with Rabbit anti-Histone H2B antibody (HA500381) at 1/2,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Lane 3: A549 cell lysate (10 µg/Lane) Lane 4: 293T cell lysate (10 µg/Lane) Lane 5: THP-1 cell lysate (10 µg/Lane) Lane 6: K562 cell lysate (10 µg/Lane) Lane 7: HL-60 cell lysate (10 µg/Lane) Lane 8: NIH/3T3 cell lysate (10 µg/Lane) Lane 9: Zebrafish tissue lysate (20 µg/Lane) Lane 10: PC-12 cell lysate (10 µg/Lane) Lane 11: RAW264.7 cell lysate (10 µg/Lane) Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500381) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - zebrafish si:dkey-23a13.8
- description:si:dkey-23a13.8
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- si:dkey-23a13.8 antibody
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