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- domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat, zebrafish , fruit fly
application: western blot, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JM10-83)
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of SDHA on different lysates with Rabbit anti-SDHA antibody (ET1703-40) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (15 µg/Lane) Lane 2: HL-60 cell lysate (15 µg/Lane) Lane 3: HeLa cell lysate (15 µg/Lane) Lane 4: MCF7 cell lysate (15 µg/Lane) Lane 5: C2C12 cell lysate (15 µg/Lane) Lane 6: L6 cell lysate (15 µg/Lane) Lane 7: Mouse brain tissue lysate (30 µg/Lane) Lane 8: Mouse heart tissue lysate (30 µg/Lane) Lane 9: Rat brain tissue lysate (30 µg/Lane) Lane 10: Rat heart tissue lysate (30 µg/Lane) Lane 11: Zebrafish tissue lysate (30 µg/Lane) Predicted band size: 73 kDa Observed band size: 70 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-40) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SDHA on different lysates with Rabbit anti-SDHA antibody (ET1703-40) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-SDHA KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 73 kDa Observed band size: 70 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-40) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling SDHA with Rabbit anti-SDHA antibody (ET1703-40) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SDHA antibody (ET1703-40) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
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gene information - zebrafish sdha
- synonym:im:7141001; zgc:56051
- description:succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
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- sdha antibody
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