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- domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of NF-κB p65 on different lysates using anti-NF-κB p65 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: PC12 cell lysate Lane 4: Mouse embryonic stem cell lysate Lane 5: NIH/3T3 cell lysate
Western blot analysis of NF-kB p65 on zebrafish lysates with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/2,000 dilution. Lysates at 20 µg/Lane. Predicted band size: 60kDa Observed band size: 70 kDa Exposure time: 3min20s; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0815) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-NF-kB p65 antibody (ER0815) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal (SZ10-04)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (ET1603-12) at 1/5,000 dilution. Lane 1: Wild-type HeLa cell lysate Lane 2: HeLa KO cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-12) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (ET1603-12) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 49 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-12) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (ET1603-12) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NF-kB p65 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-12) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (SZ10-04)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA750058) at 1/5,000 dilution. Lane 1: Wild-type HeLa cell lysate Lane 2: HeLa KO cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750058) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA750058) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 49 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750058) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NF-kB p65 on different lysates with Rabbit anti-NF-kB p65 antibody (HA750058) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NF-kB p65 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750058) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - zebrafish rela
- synonym:nfkb3; p65
- description:v-rel avian reticuloendotheliosis viral oncogene homolog A
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- rela antibody
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