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- domestic rabbit monoclonal (JE55-90)
reactivity: human, mouse, rat, zebrafish
application: western blot
Western blot analysis of MRPL28 on different lysates with Rabbit anti-MRPL28 antibody (ET7111-30) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: SW620 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: 4T1 cell lysate Lane 5: Mouse kidney tissue lysate Lane 6: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-30) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of MRPL28 on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
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gene information - zebrafish mrpl28
- synonym:wu:fa96b11; zgc:110013
- description:mitochondrial ribosomal protein L28
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product type
- mrpl28 antibody
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