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- domestic rabbit monoclonal (SU39-06)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody (ET1608-57) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: A549 cell lysate (15 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 1 minute 36 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-57) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody (ET1608-57) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate (10 µg/Lane) Lane 2: Hela-si Lactate Dehydrogenase cell lysate (10 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. ET1608-57 was shown to specifically react with Lactate Dehydrogenase in Hela-si NT cells. Weakened band was observed when Hela-si Lactate Dehydrogenase sample was tested. Hela-si NT and Hela-si Lactate Dehydrogenase samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1608-57, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
quantity: 50μl
price: 205.00 USD
to the supplier - domestic rabbit monoclonal (SU39-06)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody (HA750155) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: A549 cell lysate (15 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 1 minute 36 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750155) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Lactate Dehydrogenase on different lysates with Rabbit anti-Lactate Dehydrogenase antibody (HA750155) at 1/1,000 dilution. Lane 1: Hela-si NT cell lysate (10 µg/Lane) Lane 2: Hela-si Lactate Dehydrogenase cell lysate (10 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. ET1608-57 was shown to specifically react with Lactate Dehydrogenase in Hela-si NT cells. Weakened band was observed when Hela-si Lactate Dehydrogenase sample was tested. Hela-si NT and Hela-si Lactate Dehydrogenase samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1608-57, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750155, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - zebrafish ldhba
- synonym:LDH-B-A; ldhb; wu:fa16d07; wu:fa20c10; wu:fa96a08
- description:lactate dehydrogenase Ba
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- ldhba antibody
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