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- domestic rabbit polyclonal (3G2.2F7)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (SA39-05)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry
Western blot analysis of Ras on different lysates with Rabbit anti-Ras antibody (ET1601-16) at 1/1,000 dilution. Lane 1: 293T cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (10 µg/Lane) Lane 3: NIH/3T3 cell lysate (10 µg/Lane) Lane 4: Neuro-2a cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of MCF7 cells labeling Ras with Rabbit anti-Ras antibody (ET1601-16) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (ET1601-16) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunocytochemistry analysis of NIH/3T3 cells labeling Ras with Rabbit anti-Ras antibody (ET1601-16) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (ET1601-16) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (JF10-11)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry 
Western blot analysis of Ras on different lysates with Rabbit anti-Ras antibody (ET1702-94) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: Mouse brain tissue lysate (30 µg/Lane) Lane 5: Mouse ovary tissue lysate (30 µg/Lane) Lane 6: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Ras on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: Zebrafish tissue lysate
Immunocytochemistry analysis of HeLa cells labeling Ras with Rabbit anti-Ras antibody (ET1702-94) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ras antibody (ET1702-94) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - zebrafish kras
- synonym:K-ras; fa04e08; fc14b12; fc23g10; fj89d12; wu:fa04e08; wu:fc14b12; wu:fc23g10; wu:fj89d12; xRAS2; zgc:85725
- description:v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
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- kras antibody
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