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- mouse monoclonal (A11-B4)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of IDH1 on different lysates with Mouse anti-IDH1 antibody (EM40705) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: HEK-293 cell lysate Lane 4: A431 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: PC-12 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 42 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40705) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-IDH1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-IDH1 antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 360.00 USD
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gene information - zebrafish idh1
- synonym:cb876; fm90e09; im:7143416; wu:fm90e09
- description:isocitrate dehydrogenase (NADP(+)) 1
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- idh1 antibody
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