zebrafish hspa5 antibody | 2 products cited in the literature; 7 total from 3 suppliers

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GRP78/HSPA5 Antibody - BSA Free
Novus Biologicals
catalog: NBP1-06274

domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken, bovine, zebrafish , roundworm
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 31

Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Image shows a specific band for GRP78 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
quantity: 0.1 ml (also 0.025 ml)
price: 459 USD
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GRP78/HSPA5 Antibody (C9-9-R)
Novus Biologicals
catalog: NBP3-32408

mouse monoclonal (C9-9-R)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

GRP78/HSPA5 Antibody (C9-9-R)
quantity: 100 ul
price: 399 USD
to the supplier
Human GRP78/HSPA5 Antibody
R&D Systems
catalog: MAB4846

mouse monoclonal (474421)
reactivity: human, zebrafish
application: western blot, immunocytochemistry, immunoprecipitation
citations: 14

Detection of Human GRP78/HSPA5 antibody by Western Blot.
quantity: 100 ug (also 25 ug)
price: 469 USD
to the supplier
GRP78 / BIP
HUABIO
catalog: HA601076

total number of products: 4

mouse monoclonal (C9-9-R)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of GRP78 / BIP on different lysates with Mouse anti-GRP78 / BIP antibody (HA601076) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: Mouse brain tissue lysate (30 µg/Lane) Lane 4: U-87 MG cell lysate (30 µg/Lane) Lane 5: RAW264.7 cell lysate (30 µg/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 µg/Lane) Lane 7: Mouse liver tissue lysate (30 µg/Lane) Lane 8: Rat liver tissue lysate (30 µg/Lane) Lane 9: Rat pancreas tissue lysate (30 µg/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601076) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GRP78 / BIP antibody (HA601076) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601076) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Mouse anti-GRP78 / BIP antibody (HA601076) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601076) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
GRP78 / BIP
HUABIO
catalog: HA610013

total number of products: 4

mouse monoclonal (C9-9-R)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of GRP78 / BIP on different lysates with Mouse anti-GRP78 / BIP antibody (HA610013) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: Mouse brain tissue lysate (30 µg/Lane) Lane 4: U-87 MG cell lysate (30 µg/Lane) Lane 5: RAW264.7 cell lysate (30 µg/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 µg/Lane) Lane 7: Mouse liver tissue lysate (30 µg/Lane) Lane 8: Rat liver tissue lysate (30 µg/Lane) Lane 9: Rat pancreas tissue lysate (30 µg/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610013) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GRP78 / BIP antibody (HA610013) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610013) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Mouse anti-GRP78 / BIP antibody (HA610013) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610013) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier
GRP78 / BIP
HUABIO
catalog: M1505-13

total number of products: 4

mouse monoclonal (2-7)
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of GRP78 / BIP on different lysates with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/1,000 dilution. Lane 1: L-929 cell lysate Lane 2: U-87 MG cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate Lane 5: Mouse liver tissue lysate Lane 6: Rat liver tissue lysate Lane 7: Rat pancreas tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1505-13) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of L-929 cells labeling GRP78 / BIP with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of RAW264.7 cells labeling GRP78 / BIP with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GRP78 / BIP antibody (M1505-13) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 360.00 USD
to the supplier