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- mouse monoclonal (A11-D7)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Histone H3 on different lysates with Mouse anti-Histone H3 antibody (M1309-1) at 1/10,000 dilution and competitor's antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HT-29 cell lysate Lane 4: HEK-293 cell lysate Lane 5: C2C12 cell lysate Lane 6: L-929 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1309-1) at 1/10,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Histone H3 on zebrafish tissue lysates with Mouse anti-Histone H3 antibody (M1309-1) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1309-1) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Histone H3 on different lysates with Mouse anti-Histone H3 antibody (M1309-1) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: HepG2 cell lysate (15 µg/Lane) Lane 4: A431 cell lysate (15 µg/Lane) Lane 5: MCF7 cell lysate (15 µg/Lane) Lane 6: NIH/3T3 cell lysate (15 µg/Lane) Lane 7: C2C12 cell lysate (15 µg/Lane) Lane 8: C6 cell lysate (15 µg/Lane) Lane 9: PC-12 cell lysate (15 µg/Lane) Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1309-1) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
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gene information - zebrafish h3f3d
- synonym:His3.3B; zgc:56418
- description:H3 histone, family 3D
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product type
- h3f3d antibody
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