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- domestic rabbit monoclonal (JU11-31)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 8 - domestic rabbit monoclonal (JU11-31)
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (ET1706-45) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: U-937 cell lysate (20 µg/Lane) Lane 5: HEK-293 cell lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (20 µg/Lane) Lane 7: Rat testis tissue lysate (20 µg/Lane) Lane 8: Mouse kidney tissue lysate (20 µg/Lane) Lane 9: Rat kidney tissue lysate (20 µg/Lane) Lane 10: Mouse brain tissue lysate (20 µg/Lane) Lane 11: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-45) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GPX4 on zebrafish tissue lysates with Rabbit anti-GPX4 antibody (ET1706-45) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-45) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (ET1706-45) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: RAW264.7 cell lysate Lane 4: M-NFS-60 cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-45) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 50μl
price: 205.00 USD
to the supplier - domestic rabbit monoclonal (JU11-31)
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (HA750455) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: U-937 cell lysate (20 µg/Lane) Lane 5: HEK-293 cell lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (20 µg/Lane) Lane 7: Rat testis tissue lysate (20 µg/Lane) Lane 8: Mouse kidney tissue lysate (20 µg/Lane) Lane 9: Rat kidney tissue lysate (20 µg/Lane) Lane 10: Mouse brain tissue lysate (20 µg/Lane) Lane 11: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750455) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GPX4 on zebrafish tissue lysates with Rabbit anti-GPX4 antibody (HA750455) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750455) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (HA750455) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: Neuro-2a cell lysate Lane 3: RAW264.7 cell lysate Lane 4: M-NFS-60 cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750455) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit monoclonal (JU11-31)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
gene information - zebrafish gpx4b
- synonym:PHGPxb; cb692; zgc:110018
- description:glutathione peroxidase 4b
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- gpx4b antibody
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