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- domestic rabbit monoclonal (SC0620)
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Ferritin Heavy Chain on different lysates with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: RAW264.7 cell lysate (15 µg/Lane) Lane 3: Mouse liver tissue lysate (15 µg/Lane) Lane 4: Mouse brain tissue lysate (15 µg/Lane) Lane 5: Rat liver tissue lysate (15 µg/Lane) Lane 6: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 3 minutes 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-78) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
All lanes: Western blot analysis of Ferritin Heavy Chain with anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/5,000 dilution. Lane 1/2: Wild-type SH-SY5Y whole cell lysate (10 µg). Lane 3/4: Ferritin Heavy Chain fragment 1 knockout SH-SY5Y whole cell lysate (10 µg). Lane 5/6: Ferritin Heavy Chain fragment 1 knockout SH-SY5Y whole cell lysate (10 µg). Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-78, 1/5,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of SGC-7901 cells labeling Ferritin Heavy Chain with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 50μl
price: 205.00 USD
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gene information - zebrafish fth1b
- synonym:zgc:92245
- description:ferritin, heavy polypeptide 1b
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- fth1b antibody
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