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- domestic rabbit monoclonal (SU33-01)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 1
Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463]
quantity: 100 ul
price: 459 USD
to the supplier - domestic rabbit monoclonal (SU33-01)
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody (ET1608-25) at 1/5,000 dilution. Lane 1: THP-1 cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 70 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-25) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody (ET1608-25) at 1/2,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si FOXO1A cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 70 kDa Observed band size: 100 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-25) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling FOXO1A with Rabbit anti-FOXO1A antibody (ET1608-25) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FOXO1A antibody (ET1608-25) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (SU33-01)
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section 
Western blot analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody (HA750140) at 1/5,000 dilution. Lane 1: THP-1 cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 70 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody (HA750140) at 1/2,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si FOXO1A cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 70 kDa Observed band size: 100 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750140) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling FOXO1A with Rabbit anti-FOXO1A antibody (HA750140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FOXO1A antibody (HA750140) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation 
Western blot analysis of STAT1 (pY701) expression in HEK293T (A), NIH3T3 (B), mouse brain (C), COS7 (D) whole cell lysates.
Immunohistochemical analysis of STAT1 (pY701) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of STAT1 (pY701) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
quantity: 0.03 mL
price:
to the supplier
gene information - zebrafish foxo1b
- synonym:foxo1a.2
- description:forkhead box O1 b
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product type
- foxo1b antibody
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