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- domestic rabbit monoclonal (JA30-07)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of CaMKII delta on different lysates with Rabbit anti-CaMKII delta antibody (ET1704-37) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CaMKII delta on different lysates with Rabbit anti-CaMKII delta antibody (ET1704-37) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CaMKII delta KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: 100 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-37) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-CaMKII delta antibody (ET1704-37) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-37) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
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gene information - zebrafish camk2d1
- synonym:camk2db; wu:fc54g06; zgc:101001; zgc:173815
- description:calcium/calmodulin-dependent protein kinase (CaM kinase) II delta 1
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product type
- camk2d1 antibody
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