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- domestic rabbit monoclonal (JM10-90)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of ATPB on different lysates with Rabbit anti-ATPB antibody (ET1703-29) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: 293T cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-29) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATPB on different lysates with Rabbit anti-ATPB antibody (ET1703-29) at 1/1,000 dilution. Lane 1: RH-35 cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Mouse liver tissue lysate (40 µg/Lane) Lane 4: Mouse kidney tissue lysate (40 µg/Lane) Lane 5: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 57 kDa Observed band size: 50 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-29) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATPB on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
gene information - zebrafish atp5f1b
- synonym:atp5b; fj13e04; fj55c09; hm:zehn0534; im:6793121; wu:fj13e04; wu:fj38d01; wu:fj55c09; zgc:111961
- description:ATP synthase F1 subunit beta
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product type
- atp5f1b antibody
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