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- domestic rabbit monoclonal (JM102-06)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Immunohistochemistry-Paraffin: beta-2 Adrenergic R/ADRB2 Antibody (JM102-06) [NBP2-67187]
quantity: 100 ul
price: 449 USD
to the supplier - domestic rabbit monoclonal (JM102-06)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Immunofluorescence analysis of frozen mouse liver tissue with Rabbit anti-beta 2 Adrenergic Receptor antibody (ET1703-04) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-04, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Western blot analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody (ET1703-04) at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: Human liver tissue lysate (20 µg/Lane) Lane 3: Mouse heart tissue lysate (20 µg/Lane) Lane 4: Rat heart tissue lysate (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 46 kDa Observed band size: 55-100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-04) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody (ET1703-04) at 1/1,000 dilution. Lane 1: A431 cell lysate (no heat) (15 µg/Lane) Lane 2: Mouse kidney tissue lysate (no heat) (20 µg/Lane) Lane 3: Mouse heart tissue lysate (no heat) (20 µg/Lane) Lane 4: Rat kidney tissue lysate (no heat) (20 µg/Lane) Lane 5: Zebrafish tissue lysate (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 46 kDa Observed band size: 55-100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (JM102-06)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Immunofluorescence analysis of frozen mouse liver tissue with Rabbit anti-beta 2 Adrenergic Receptor antibody (HA750368) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750368, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Western blot analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody (HA750368) at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: Human liver tissue lysate (20 µg/Lane) Lane 3: Mouse heart tissue lysate (20 µg/Lane) Lane 4: Rat heart tissue lysate (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 46 kDa Observed band size: 55-100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750368) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of beta 2 Adrenergic Receptor on different lysates with Rabbit anti-beta 2 Adrenergic Receptor antibody (HA750368) at 1/1,000 dilution. Lane 1: A431 cell lysate (no heat) (15 µg/Lane) Lane 2: Mouse kidney tissue lysate (no heat) (20 µg/Lane) Lane 3: Mouse heart tissue lysate (no heat) (20 µg/Lane) Lane 4: Rat kidney tissue lysate (no heat) (20 µg/Lane) Lane 5: Zebrafish tissue lysate (20 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 46 kDa Observed band size: 55-100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750368) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
gene information - zebrafish adrb2a
- description:adrenoceptor beta 2, surface a
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- adrb2a antibody
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