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- domestic rabbit polyclonal
reactivity: western clawed frog, human, mouse, rat
application: western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
Figure 1. Western blot analysis of MED18 using anti-MED18 antibody (A10600-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, . Lane 2: human K562 whole cell lysates,. Lane 3: human U2OS whole cell lysates,. Lane 4: human HL-60 whole cell lysates,. Lane 5: rat liver tissue lysates,. Lane 6: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED18 antigen affinity purified polyclonal antibody (Catalog # A10600-2) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED18 at approximately 24KD. The expected band size for MED18 is at 24KD.
Figure 2. IHC analysis of MED18 using anti-MED18 antibody (A10600-2). MED18 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (A10600-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of MED18 using anti-MED18 antibody (A10600-2). MED18 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED18 Antibody (A10600-2) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
quantity: 100 ug/vial
price: 315 USD
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gene information - western clawed frog med18
- description:mediator complex subunit 18
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- med18 antibody
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