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- domestic rabbit polyclonal
reactivity: thale cress
application: immunohistochemistry - paraffin section
Immunofluorescence staining of paraffin- embedded Arabidopsis thaliana using anti-PAS2 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-29 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1/500 for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Arabidopsis thaliana tissue using anti-PAS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - thale cress PAS2
- synonym:F12B17.170; F12B17_170; PASTICCINO 2; PEP; PEPINO
- description:Protein-tyrosine phosphatase-like, PTPLA
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product type
- PAS2 antibody
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