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- domestic rabbit polyclonal
reactivity: roundworm
application: western blot
citations: 5
Western blot analysis of Alg-1 was performed by loading 15µg WT or Alg-1 (-) worm extract onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing Alg-1 (Product # PA1-031) at a dilution of 1:5000 for 1 hour and room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product #31460) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico (Product #34077).
quantity: 100 ug
price: US 451.00
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gene information - roundworm alg-1
- description:Protein argonaute
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- alg-1 antibody
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