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- domestic rabbit monoclonal (PSH17-84)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of Polymeric immunoglobulin receptor / PIGR on different lysates with Rabbit anti-Polymeric immunoglobulin receptor / PIGR antibody (HA723953) at 1/5,000 dilution. Lane 1: A431 cell lysate Lane 2: A549 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 83 kDa Observed band size: 70-100 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723953) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Polymeric immunoglobulin receptor / PIGR on different lysates with Rabbit anti-Polymeric immunoglobulin receptor / PIGR antibody (HA723953) at 1/5,000 dilution. Lane 1: Mouse colon tissue lysate Lane 2: Rat stomach tissue lysate Lane 3: Rat colon tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 83 kDa Observed band size: 70-100 kDa Exposure time: 42 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723953) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Polymeric immunoglobulin receptor / PIGR was immunoprecipitated from 0.2 mg A431 cell lysate with HA723953 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723953 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA723953 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA723953 in A431 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801
quantity: 100μl
price: 385.00 USD
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gene information - rat polymeric immunoglobulin receptor
- synonym:RNPIGR2; pIgA-R
- description:polymeric immunoglobulin receptor
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product type
- polymeric immunoglobulin receptor antibody
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