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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18 ...
Abcam
catalog: ab184743domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of PLCB3 on different lysates with Rabbit anti-PLCB3 antibody (HA500391) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: RAW264.7 cell lysate (10 µg/Lane) Lane 3: SH-SY5Y cell lysate (10 µg/Lane) Lane 4: N2A cell lysate (10 µg/Lane) Lane 5: Mouse cerebellum tissue lysate (20 µg/Lane) Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500391) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PLCB3 antibody (HA500391) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500391) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PLCB3 antibody (HA500391) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500391) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
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gene information - rat phospholipase C beta 3
- description:phospholipase C beta 3
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product type
- phospholipase C beta 3 antibody
- phospholipase C beta 3 ELISA/assay
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