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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
Western blot analysis of GAMT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500071, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate
Immunocytochemistry analysis of HeLa cells labeling GAMT with Rabbit anti-GAMT antibody (HA500071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GAMT antibody (HA500071) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier - Rabbit anti-human guanidinoacetate N-methyltransferase polyclonal Antibody
MyBioSource
catalog: MBS711698domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
gene information - rat guanidinoacetate methyltransferase
- synonym:GMT
- description:guanidinoacetate N-methyltransferase
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product type
- guanidinoacetate methyltransferase antibody
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