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- domestic rabbit monoclonal (PSH11-67)
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of EEF2K on different lysates with Rabbit anti-EEF2K antibody (HA723363) at 1/10,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: Mouse ovary tissue lysate (40 µg/Lane) Lane 7: Rat ovary tissue lysate (40 µg/Lane) Predicted band size: 82 kDa Observed band size: 82-90 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723363) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of EEF2K on different lysates with Rabbit anti-EEF2K antibody (HA723363) at 1/5,000 dilution. Lane 1: HeLa-parental cell lysate Lane 2: HeLa-EEF2K KD cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 82 kDa Observed band size: 82-90 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723363) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling EEF2K with Rabbit anti-EEF2K antibody (HA723363) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EEF2K antibody (HA723363) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH11-67)
reactivity: human, mouse, rat
application: western blot, flow cytometry 
Western blot analysis of EEF2K on different lysates with Rabbit anti-EEF2K antibody (HA751423) at 1/10,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: Mouse ovary tissue lysate (40 µg/Lane) Lane 7: Rat ovary tissue lysate (40 µg/Lane) Predicted band size: 82 kDa Observed band size: 82-90 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751423) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of EEF2K on different lysates with Rabbit anti-EEF2K antibody (HA751423) at 1/5,000 dilution. Lane 1: HeLa-parental cell lysate Lane 2: HeLa-EEF2K KD cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 82 kDa Observed band size: 82-90 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751423) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling EEF2K with Rabbit anti-EEF2K antibody (HA751423) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EEF2K antibody (HA751423) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken
application: western blot, immunocytochemistry, immunoprecipitation - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation, reverse phase protein lysate microarray, other, western blot knockout validation
citations: 4
gene information - rat eukaryotic elongation factor 2 kinase
- synonym:SMEF2K
- description:eukaryotic elongation factor-2 kinase
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product type
- eukaryotic elongation factor 2 kinase antibody
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