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- domestic goat polyclonal
reactivity: human, mouse, rat, dogs, bovine
application: western blot, enzyme immunoassay - domestic rabbit monoclonal (JE50-74)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of NUDC on different lysates with Rabbit anti-NUDC antibody (ET7110-18) at 1/5,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Rat testis tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 38 kDa Observed band size: 45 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-18) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NUDC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysates Lane 2: PC-3M cell lysates Lane 3: U937 cell lysates
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NUDC antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-18, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
gene information - rat clone 15
- synonym:Silg92; c15
- description:nuclear distribution C, dynein complex regulator
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product type
- clone 15 antibody
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