Your Filters
product
- domestic rabbit polyclonal
reactivity: human, mouse, rat, dogs, chimpanzee
application: western blot, ELISA
Western blot using Rockland's Affinity Purified anti-ZIC2 antibody shows detection of a band ~55 kDa (arrowhead) corresponding to ZIC2 in lysates from (lane 1) mouse brain and (lane 2) rat brain. Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-ZIC2 antibody. Signal was detected using standard techniques. The upper band in the mouse brain lysate represents Zic2.
quantity: 100 µg
price:
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section 
Western blot analysis of Zic2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500195, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human brain tissue lysate Lane 2: Rat cerebellum tissue lysate
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Zic2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500195, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Zic2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500195, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
gene information - rat Zic2
- description:Zic family member 2
do you know?
- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Zic2 antibody
species
linkout
questions and comments