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- domestic rabbit monoclonal (JE63-65)
reactivity: human, rat
application: western blot
Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/1,000 dilution. Lane 1: HUVEC cell lysate Lane 2: HeLa cell lysate Lane 3: SiHa cell lysate Lane 4: Jurkat cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 156 kDa Observed band size: 156 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/500 dilution. Lane 1: HUVEC cell lysate, 10 µg/Lane Lane 2: Rat brain tissue lysate, 20 µg/Lane Predicted band size: 156 kDa Observed band size: 156 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
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gene information - rat Zfyve9
- description:zinc finger FYVE-type containing 9
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- Zfyve9 antibody
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