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- domestic rabbit monoclonal (PSH0-39)
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of WNT8A on different lysates with Rabbit anti-WNT8A antibody (HA721341) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 µg/Lane) Lane 2: HepG2 cell lysate (30 µg/Lane) Lane 3: SW480 cell lysate (30 µg/Lane) Lane 4: Jurkat cell lysate (30 µg/Lane) Lane 5: Raji cell lysate (30 µg/Lane) Lane 6: NIH/3T3 cell lysate (30 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Lane 8: Mouse embryo tissue lysate (40 µg/Lane) Lane 9: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 38.8 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721341) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of NIH/3T3 cells labeling WNT8A with Rabbit anti-WNT8A antibody (HA721341) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-WNT8A antibody (HA721341) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Flow cytometric analysis of HeLa cells labeling WNT8A. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721341, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal (PSH0-39)
reactivity: human, mouse, rat
application: western blot, flow cytometry 
Western blot analysis of WNT8A on different lysates with Rabbit anti-WNT8A antibody (HA750617) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 µg/Lane) Lane 2: HepG2 cell lysate (30 µg/Lane) Lane 3: SW480 cell lysate (30 µg/Lane) Lane 4: Jurkat cell lysate (30 µg/Lane) Lane 5: Raji cell lysate (30 µg/Lane) Lane 6: NIH/3T3 cell lysate (30 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Lane 8: Mouse embryo tissue lysate (40 µg/Lane) Lane 9: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 38.8 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750617) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of NIH/3T3 cells labeling WNT8A with Rabbit anti-WNT8A antibody (HA750617) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-WNT8A antibody (HA750617) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Flow cytometric analysis of HeLa cells labeling WNT8A. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750617, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, rat
application: immunohistochemistry - paraffin section
gene information - rat Wnt8a
- description:Wnt family member 8A
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product type
- Wnt8a antibody
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