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- rat monoclonal (442625)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, neutralization, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, western blot knockout validation
citations: 44
Detection of Human and Mouse Wnt-5a by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c.) tissue. PVDF Membrane was probed with 2 ╡g/mL of Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005 ). A specific band was detected for Wnt-5a at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
quantity: 100 ug (also 25 ug)
price: 489 USD
to the supplier - domestic goat polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, neutralization, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 27 
Wnt-5a in Mouse Embryonic Rib. Wnt-5a was detected in immersion fixed paraffin-embedded sections of mouse embryonic rib using 15 ug/mL Mouse/Rat Wnt-5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) overnight at 4 ░C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008 ) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .
quantity: 100 ug (also 25 ug)
price: 489 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 16 - domestic rabbit polyclonal
reactivity: human, rat
application: western blot - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry 
Immunohistochemistry of Rabbit Anti-Wnt5A antibody. Tissue: human breast tumor. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Wnt5A antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Wnt5A is a secreted in the extracellular space and matrix. Staining: Wnt5A as a precipitated red signal with hematoxylin purple nuclear counterstain.
Western Blot of Rabbit Anti-Wnt5A antibody. Lane 1: heart lysate without immunizing peptide. Lane 2: heart lysate with immunizing peptide. Lane 3: mouse. Lane 4: rat heart. Primary antibody: Wnt5A at 0.5 µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 80 kDa for Wnt5A. Other band(s): none.
quantity: 100 µg
price:
to the supplier
gene information - rat Wnt5a
- description:Wnt family member 5A
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- we limit the listing of re-branded antibodies to provide our visitors with accurate information and the best experience
- we manually curate antibody information from the literature to obtain a comprehensive set of well-validated antibodies.
- we limit citation information for polyclonal antibodies to articles published within the last 5 years because the supply of a particular polyclonal antibody preparation is limited.
product type
- Wnt5a antibody
- Wnt5a ELISA/assay
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