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- mouse monoclonal (A7C2)
reactivity: human, mouse, rat
application: western blot
Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/20,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 62 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/5,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling WLS with Mouse anti-WLS antibody (HA600060) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-WLS antibody (HA600060) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 360.00 USD
to the supplier - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, enzyme immunoassay 
mouse liver tissue were subjected to SDS PAGE followed by western blot with GPRI77 antibody at dilution of 1:400
Immunohistochemical of paraffin-embedded human ovary using GPR177 antibody at dilution of 1 :100 (under lOx lens)
Immunohistochemical of paraffm-embedded human ovary using GPR177 antibody at dilution of 1: 1 00 (under 40x lens)
quantity: 0.06 mL
price:
to the supplier
gene information - rat Wls
- synonym:Gpr177
- description:Wnt ligand secretion mediator
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product type
- Wls antibody
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