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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of WASL on different lysates with Rabbit anti-WASL antibody (ER1803-16) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-WASL KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 70 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-16) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of WASL on different lysates with Rabbit anti-WASL antibody (ER1803-16) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 65 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-16) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of MCF7 cells labeling WASL with Rabbit anti-WASL antibody (ER1803-16) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WASL antibody (ER1803-16) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier - domestic rabbit monoclonal (30D10)
reactivity: human, mouse, rat, dogs, bovine
application: western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, western blot knockout validation, immunohistochemistry knockout validation
citations: 27
gene information - rat Wasl
- synonym:N-WASP; TRS4
- description:WASP like actin nucleation promoting factor
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product type
- Wasl antibody
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