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- domestic rabbit monoclonal (JG40-19)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of VAMP1 on different lysates with Rabbit anti-VAMP1 antibody (ET7109-03) at 1/500 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 13 kDa Observed band size: 15 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-03) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 385.00 USD
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gene information - rat Vamp1
- synonym:Syb1
- description:vesicle-associated membrane protein 1
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product type
- Vamp1 antibody
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