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- domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, immunoprecipitation - domestic rabbit polyclonal (CL5339)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section - mouse monoclonal (A1F9)
reactivity: human, rat
application: western blot
Western blot analysis of USP28 on different lysates with Mouse anti-USP28 antibody (EM1901-46) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: 293T cell lysate Lane 4: SW480 cell lysate Lane 5: LNCaP cell lysate Lane 6: MDA-MB-231 cell lysate Lane 7: MDA-MB-468 cell lysate Lane 8: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 122 kDa Observed band size: 140 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-46) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
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gene information - rat Usp28
- description:ubiquitin specific peptidase 28
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product type
- Usp28 antibody
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