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- domestic rabbit monoclonal (JU63-27)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of USP22 on different lysates with Rabbit anti-USP22 antibody (ET1706-13) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: Jurkat cell lysate (15 µg/Lane) Lane 4: MCF7 cell lysate (15 µg/Lane) Lane 5: Neuro-2a cell lysate (15 µg/Lane) Lane 6: F9 cell lysate (15 µg/Lane) Lane 7: C6 cell lysate (15 µg/Lane) Lane 8: PC-12 cell lysate (15 µg/Lane) Lane 9: Human brain tissue lysate (30 µg/Lane) Lane 10: Human liver tissue lysate (30 µg/Lane) Lane 11: Mouse brain tissue lysate (30 µg/Lane) Lane 12: Rat brain tissue lysate (30 µg/Lane) Lane 13: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: Lane 1-3: 6 seconds; Lane 4-13: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-13) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of USP22 on different lysates with Rabbit anti-USP22 antibody (ET1706-13) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-USP22 KD cell lysate (10 µg/Lane) Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 60 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-13) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-USP22 antibody (ET1706-13) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-13) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
gene information - rat Usp22
- description:ubiquitin specific peptidase 22
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- Usp22 antibody
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