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- domestic rabbit polyclonal (MEM-258)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section - domestic rabbit monoclonal (JE34-69)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation
Western blot analysis of CRMP5 on different lysates with Rabbit anti-CRMP5 antibody (HA722782) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Mouse testis tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Lane 5: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722782) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
CRMP5 was immunoprecipitated from 0.2 mg SH-SY5Y cell lysate with HA722782 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722782 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SH-SY5Y cell lysate (input) Lane 2: HA722782 IP in SH-SY5Y cell lysate Lane 3: Rabbit IgG instead of HA722782 in SH-SY5Y cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds; ECL: K1802
quantity: 100μl
price: 385.00 USD
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gene information - rat Ulip6
- synonym:Crmp5; Ulip6
- description:dihydropyrimidinase-like 5
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product type
- Ulip6 antibody
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