UFC1
HUABIO
catalog: HA721111

domestic rabbit monoclonal (JE65-12)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of UFC1 on different lysates with Rabbit anti-UFC1 antibody (HA721111) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-UFC1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721111) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of UFC1 on 293T cell lysates with Rabbit anti-UFC1 antibody (HA721111) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721111) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SH-SY5Y cells labeling UFC1 with Rabbit anti-UFC1 antibody (HA721111) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-UFC1 antibody (HA721111) at 1/400 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) were used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier

Anti-UFC1 antibody [EPR15014]
Abcam
catalog: ab189251

domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

quantity: 100µL, 1 mL
price:
to the supplier

Ufc1 Antibody - BSA Free
Novus Biologicals
catalog: NBP1-81676

domestic rabbit polyclonal (OTI1A11)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 0.1 ml (also 25 ul)
price: 499 USD
to the supplier

Ufc1 Antibody - BSA Free
Novus Biologicals
catalog: NBP1-81677

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 0.1 ml (also 25 ul)
price: 499 USD
to the supplier