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- domestic rabbit monoclonal (JE55-76)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of UBE4B on different lysates with Rabbit anti-UBE4B antibody (ET7111-11) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: Raji cell lysate (10 µg/Lane) Lane 3: RAW264.7 cell lysate (10 µg/Lane) Lane 4: PC-12 cell lysate (10 µg/Lane) Lane 5: Mouse testis tissue lysate (20 µg/Lane) Lane 6: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 146 kDa Observed band size: 146 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-11) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
All lanes: Western blot analysis of UBE4B with anti-UBE4B antibody [JE55-76] (ET7111-11) at 1:1,000 dilution. Lane 1: Wild-type N2A whole cell lysate (30 µg). Lane 2: UBE4B knockout N2A whole cell lysate (30 µg). ET7111-11 was shown to specifically react with UBE4B in wild-type N2A cells. No band was observed when UBE4B knockdout sample was tested. Wild-type and UBE4B knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7111-11, 1:1,000) was used in 5% BSA at room temperature for 2 hours.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-UBE4B antibody (ET7111-11) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-11) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit monoclonal
reactivity: human, mouse, rat
application: western blot - domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
gene information - rat Ube4b
- synonym:ufd2a
- description:ubiquitination factor E4B
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- Ube4b antibody
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